文章摘要
袁荣高,杨留才,刘德军,李仕红,徐红涛.淋巴细胞中pot1 mrna表达?端粒酶活性与2型糖尿病发生发展的关系[J].南京医科大学学报,2011,(4):483~486
淋巴细胞中pot1 mrna表达?端粒酶活性与2型糖尿病发生发展的关系
relationship of pot1 mrna expression,telomerase activity and development of type 2 diabetes
投稿时间:2010-10-06  
DOI:10.7655
中文关键词: pot1 mrna  端粒酶活性  2型糖尿病  血糖控制  视网膜病变分期  病程
英文关键词: pot1 mrna  telomerase activity  type 2 diabetic mellitus  glycemic control  retinopathy stage  course
基金项目:盐城市科技发展计划项目(YK20080121)
作者单位
袁荣高 连云港中医药高等职业技术学校,江苏 连云港 222006 
杨留才 盐城卫生职业技术学院,江苏 盐城 224006 
刘德军 连云港中医药高等职业技术学校,江苏 连云港 222006 
李仕红 盐城卫生职业技术学院,江苏 盐城 224006 
徐红涛 盐城卫生职业技术学院,江苏 盐城 224006 
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中文摘要:
      目的:研究淋巴细胞中端粒保护蛋白(pot1)的表达?端粒酶活性与2型糖尿病发生发展的关系?方法:以2型糖尿病患者162例为实验组,以健康体检者162例为对照组,rt-pcr检测pot1 mrna的表达;trap-elisa分析端粒酶活性?结果:实验组pot1 mrna的表达和端粒酶活性均明显高于对照组?其中血糖控制尚可组和差组分别与对照组比较,pot1 mrna表达和端粒酶活性均明显增高;按糖尿病性视网膜病变分期中各组pot1 mrna表达和端粒酶活性与对照组比较存在显著性差异;且病程5~10年组?10年以上组与5年以下组相比,pot1 mrna表达和端粒酶活性也存在显著性差异?结论:2型糖尿病淋巴细胞中pot1 mrna的表达和端粒酶活性均明显增高,且与病情发展呈正相关?pot1和端粒酶的表达变化作为预后的评价依据有重要的临床意义?
英文摘要:
      objective:to study the relationship of protection of telomeres 1(pot1) mrna expression,telomerase activity in lymphocytes with the development of type 2 diabetic mellitus(t2dm). methods:one hundred and sixty-two cases with t2dm were test group,162 cases of healthy were control group,real-time quantitative pcr was used to analysis pot1 mrna expression,semi-quantitative trap-elisa was used to analysis telomerase activity. results:the expression of pot1 mrna and telomerase relatively activity in test group were obviously higher than those in control group(p < 0.01). meanwhile,the pot1 mrna expression and telomerase activity were significantly higher in t2dm patients with passable or poor controlled blood glucose compared with controls(p < 0.01),and they were also significantly higher in t2dm patients with different stage of diabetic retinopathy compared with controls(p < 0.01),significant differences also existed among the patients whose time course of t2dm between 5 to 10 years or above 10 years and time course of t2dm less than 5 years or controls(p < 0.01). conclusion:pot1 mrna expression and the telomerase activity were significantly increased with the progression of t2dm;they might be the important evidences in disease prognosis.
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