objective:to analyze hla-a,b and drb1 alleles high-resolution polymorphism and haplotypes in the han population in jiangsu province. methods:pcr-ssop and pcr-sbt methods were applied for hla high-resolution genotyping of 644 unrelated healthy donors in jiangsu branch of chinese national marrow donor program (cmdp) registry. the allele frequencies at hla-a,b,and drb1 loci were estimated by direct counting method and the haplotype frequencies were estimated using arlequin software 3.01. results:a total of 29 alleles for hla-a,60 alleles for hla-b,and 36 alleles for hla-drb1 were found. the most common alleles were a*1101(17.86%),b*4601(9.55%)and drb1*0901(15.76%). the most common a-b-drb1 haplotype was a*3001-b*1302-drb1*0701(5.98%),the most common a-b haplotype was a*3001-b*1302(6.68%),the most common b-drb1 haplotype was b*1302-drb1*0701(7.22%)and the most common a-drb1 haplotype was a*3001-drb1*0701(6.06%). conclusion:the high-resolution results show the characteristics of hla distribution in jiangsu han population,and it is helpful in hla matching in transplantation,as well as to population genetics and disease association studies.