| 汤 郁,陆 化,张广莲,陆益龙,胡慧瑾,李俊霞,吴雨洁,王丽霞,费小明.与骨髓瘤细胞共培养时对骨髓间充质细胞表达DKK1和HGF的影响[J].南京医科大学学报,2011,(5):612~615 |
| 与骨髓瘤细胞共培养时对骨髓间充质细胞表达DKK1和HGF的影响 |
| The “cross-talk” between bone marrow derived mesenchymal stem cells (MSCs) and U266 cell line dysregulated the expression of DKK1 and HGF in MSCs |
| 投稿时间：2010-12-21 |
| DOI：10.7655 |
| 中文关键词: 骨髓瘤 间充质干细胞 Dickkopf 1 肝细胞生长因子 骨髓微环境 |
| 英文关键词: multiple myeloma mesenchymal stem cells DKK1 HGF bone marrow microenvironment |
| 基金项目:江苏省自然科学基金（BK2008236);江苏省高校自然科学基础研究面上项目（07KJB320074) |
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| 中文摘要: |
| 目的:研究缺铁性贫血患者骨髓间充质干细胞(mesenchymal stem cells,MSCs)在与骨髓瘤细胞株U266共培养过程中,骨髓瘤细胞对MSCs DKK1和HGF表达的影响?方法:将体外培养的骨髓MSCs,在Transwell的条件下与骨髓瘤细胞株U266共培养后,检测骨髓MSCs的生长?培养液上清中肿瘤坏死因子-α(TNF-α)的水平,以及Dickkopf 1(DKK1)和肝细胞生长因子(hepatocyte growth facfor,HGF)表达的变化? 结果:骨髓MSCs与U266共培养后,与对照组MSCs比较其形态和生长未见明显改变,TNF-α的水平也没有明显升高,但经Reat-time PCR检测,骨髓MSC的DKK1和HGF mRNA表达水平有改变,其中在共培养第5天时间点DKK1和HGF均增高(P < 0.05),但在第8和12天2个时间点没有统计学差异(P > 0.05)?结论:骨髓MSCs在与U266骨髓瘤细胞共培养后,U266诱导骨髓MSCs的HGF和DKK1表达出现异常? |
| 英文摘要: |
| Objective:To ivestigate the possible effects on normal bone marrow derived mesenchymal stem cells(MSCs) when co-cultured with myeloma cell line U266. Methods:Bone marrow MSCs from normal subjects were co-cultured with U266 by transwell. On the given time point, the levels of TNF-α in the medium, the mRNA expression levels of Dickkopf 1(DKK1) and hepatocyte growth factor(HGF) were mesured. Results:No evident morphologic and proliferative alterations could be observed in bone marrow derived MSCs after co-cultured with U266, and there was also no detectable increase in the level of TNF-α in superant of the co-culture system. However, the mRNA expression levels of DKK1 and HGF were upregulated in bone marrow MSCs after 5 days’ co-culture with U266. Conlusion:The “cross-talk” between bone marrow MSCs and U266 induced dysregulation of DKK1 and HGF, which might be involved in the pathogenesis of multiple myeloma. |
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