文章摘要
夏 梦,刘明兮,周 涛,何 辉,万 茹,霍 然.小鼠生发泡期卵母细胞成熟过程中转录沉默机制的探讨[J].南京医科大学学报,2011,(7):956~961
小鼠生发泡期卵母细胞成熟过程中转录沉默机制的探讨
The explore of the mechanism of transcriptional silencing during germinal vesicle oocytes maturation in mice
投稿时间:2011-01-21  
DOI:10.7655
中文关键词: 生发泡卵母细胞  转录沉默  RNA聚合酶Ⅱ  组蛋白H3K4/K9三甲基化
英文关键词: germinal vesicle oocyte  transcription silencing  RNA PolymeraseⅡ  H3K4/H3K9me3
基金项目:国家自然科学基金(30700275)
作者单位
夏 梦 南京医科大学组织胚胎学系,江苏 南京 210029 
刘明兮 南京医科大学组织胚胎学系,江苏 南京 210029 
周 涛 南京医科大学组织胚胎学系,江苏 南京 210029 
何 辉 南京医科大学组织胚胎学系,江苏 南京 210029 
万 茹 南京医科大学组织胚胎学系,江苏 南京 210029 
霍 然 南京医科大学组织胚胎学系,江苏 南京 210029 
摘要点击次数: 1858
全文下载次数: 133
中文摘要:
      目的:探讨小鼠生发泡卵母细胞成熟过程中发生大规模转录沉默的机制?方法:选用4~6周龄ICR雌性小鼠,分离生发泡(germinal vesicle, GV)期卵母细胞,运用RNA聚合酶Ⅱ抗体(anti-RNA PolⅡCTD, clone 4H8)及其羧基末端结构域(CTD)基团第2位丝氨酸磷酸化的抗体(anti-RNA PolⅡCTD Ser2-P, clone H5)?组蛋白H3K4/H3K9三甲基化的抗体(anti-H3K4me3?anti-H3K9me3)进行免疫荧光染色?观察伴随GV期小鼠卵母细胞染色质构型由非包围核仁(non-surround nucleolus,NSN)型转变为包围核仁(surround nucleolus, SN)型的过程,这两对标记物的表达及分布变化?结果:在NSN型的GV期卵母细胞中,RNA PolⅡCTD Ser2-P于核浆中呈特征性斑块状分布,同时H3K4me3在核浆中也显示强阳性信号;但在SN型卵母细胞中,RNA PolⅡCTD Ser2-P失去特征性分布,转变为核浆中弥漫性分布,H3K4me3的核浆中阳性信号也消失?结论:RNA聚合酶Ⅱ的活性降低及组蛋白修饰状态的改变在小鼠GV期卵母细胞成熟过程的大规模转录沉默中发挥了重要作用?
英文摘要:
      Objective: To explore the mechanism of large-scale transcriptional silence during germinal vesicle (GV) oocytes maturation in mice. Methods:The separated GV oocytes were obtained from 4~6-week-old ICR female mice. As for the observation about the expressional and distributional changes of both markers in the process of the chromatin configuration from non-surround nucleolus(NSN) type into surround nucleolus(SN) type in mice GV oocytes, these oocytes were immunofluorescence stained with RNA polymeraseⅡ antibody(anti-RNA PolⅡCTD, clone 4H8) and the second serine phosphorylated antibody at RNA polymeraseⅡCTD group (anti-RNA PolⅡCTD Ser2-P, clone H5); as well as histone H3K4/H3K9 tri-methylation antibodies (anti-H3K4me3, anti-H3K9me3). Results:In NSN type GV oocytes, RNA CTD PolⅡSer2-P was distributed with characteristic plaques shape in nucleus; meanwhile, H3K4me3 also presented the strong positive signals in nucleus. However, in SN type oocytes, RNA CTD PolⅡSer2-P lost the characteristic distribution and diffused in nucleus; moreover, the positive signal in nucleus showed by H3K4me3 disappeared. Conclusion:The reduction of RNA polymeraseⅡactivity and the change of histone modification status played an important role in the large-scale transcriptional silencing during germinal vesicle oocytes maturation of mice.
查看全文   查看/发表评论  下载PDF阅读器