Objective:To investigate the effects of fluvastatin and losartan on vascular endothelial growth factor(VEGF) biosynthesis by human podocyte treated with angiotensin Ⅱ(AngⅡ). Methods:Cultured human podocytes were incubated with AngⅡfor 24 h at the concentration from 10-9 to 10-5 mol/L to choose the suitable concentration,and the optimal treatment time were chosed at the AngⅡ concentration of 10-7 mol/L after treated different times(0,6,12 and 24 hours). The podocytes were incubated with AngⅡ(10-7 mol/L) and different concentrations of fluvastatin(10-7~10-5 mol/L) for 24 h to choose the suitable concentration of fluvastatin. Then the podocytes were incubated with AngⅡ(10-7 mol/L) and different concentrations of losartan(10-6~10-4 mol/L) for 24 h to choose the suitable concentration of losartan. Finally,the podocytes were incubated by AngⅡ(10-7 mol/L) with fluvastatin and losartan(both 10-6 mol/L),and VEGF expression was detected using Western bolt. Results:①VEGF synthesis by human podocyte cultured in AngⅡ medium increased obviously at the concentration of 10-7 mol/L after 24 h. ②Fluvastatin or losartan inhibited VEGF expression stimulated by AngⅡ,and the 10-5 mol/L of fluvastatin and the 10-4 mol/L of losartan had the most siginificant effect. ③Fluvastatin(10-6 mol/L) combined with losartan(10-6 mol/L) inhibited VEGF expression more siginificantly than the effect of each alone. Conclusion:It is suggested that AngⅡ stimulates VEGF biosynthesis by human podocyte in a time and concentration pattern. Both fluvastatin and losartan can inhibit this effect,and they have synergistic inhibitory effects.