文章摘要
李昕昕,冯娇娇,侯道荣,郭 静,王学军,王 宁,袁 栎.IRE1α 通过UPR 影响非洲爪蟾胚胎发育[J].南京医科大学学报,2011,(10):1399~1402
IRE1α 通过UPR 影响非洲爪蟾胚胎发育
The role of IRE1α on embryo development of xenopus lavies
投稿时间:2011-07-14  
DOI:10.7655
中文关键词: IRE1α  非洲爪蟾  胚胎发育
英文关键词: IRE1α  xenopus laevis  embryo development
基金项目:国家自然科学基金资助(30971680)
作者单位
李昕昕 南京医科大学生化与分子生物学系,江苏 南京 210029 
冯娇娇 南京医科大学生化与分子生物学系,江苏 南京 210029 
侯道荣 南京医科大学生化与分子生物学系,江苏 南京 210029 
郭 静 南京医科大学生化与分子生物学系,江苏 南京 210029 
王学军 南京医科大学生化与分子生物学系,江苏 南京 210029 
王 宁 南京医科大学生化与分子生物学系,江苏 南京 210029 
袁 栎 南京医科大学生化与分子生物学系,江苏 南京 210029 
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中文摘要:
      目的:探讨IRE1α在非洲爪蟾胚胎发育中是否介导未折叠蛋白反应(UPR),并在衣霉素(tunicamycin,TM)诱导的胚胎发育畸形中发挥挽救作用?方法:利用TM处理胚胎激发内质网应激;应用显微注射mRNA方法实现基因过表达,通过注射特异的反义寡核苷酸morpholino(MO)实现基因封闭;RT-PCR检测XBP1 剪切情况?结果:XBP1 剪切随IRE1α过表达及封闭而增加或减少;TM处理导致胚胎发育畸形,XBP1剪切增加,封闭XBP1可部分挽救发育畸形;封闭IRE1a可明显挽救发育畸形,XBP1剪切恢复?结论:IRE1α在非洲爪蟾胚胎发育中介导UPR,封闭IRE1α可逆转TM诱导的胚胎发育畸形,部分通过XBP1途径发挥作用?
英文摘要:
      Objective: To explore whether IRE1α can mediate unfolded protein response (UPR) during embryo development of xenopus laevis and rescue the developmental defects induced with tunicamycin. Methods: IRE1α mRNA or specific antisense oligonucleotids morpholino were microinjected to the 4 blastomeres of 4-cell stage embyos to overexpress or knockdown gene expression;the splicing of XBP1 was tested with RT-PCR to reflect the UPR;Tunicamycin was used to treat the embryos to induce the endoplasmic reticulum stress (ERS). Results: The splicing of XBP1 was increased when IRE1α was overexpressed while the splicing was decreased when IRE1α was knockdwon. Embryos developmental defects and the increase of XBP1 splicing were induced with tunicamycin while knockdown of XBP-1 partially rescued the defects and knockdown of IRE1α rescued the defects significantly and the increased XBP1 splicing. Conclusion: IRE1α can mediate UPR during xenopus development. Knockdown of IRE1α rescued the developmental defects induced with TM and partially by the IRE1α/XBP1 pathway.
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