文章摘要
刘 娟,王 龙,胡 淼,丁国宪.小鼠棕色脂肪原代培养模型的建立[J].南京医科大学学报,2011,(12):1752~1755
小鼠棕色脂肪原代培养模型的建立
Establishment of culture model for primary mouse brown adipocyte precursor cells
投稿时间:2011-09-27  
DOI:10.7655
中文关键词: 前棕色脂肪细胞  原代培养  诱导分化
英文关键词: brown adipocyte  primary cell culture  cell differentiation
基金项目:国家自然科学基金资助(30900504)
作者单位
刘 娟 南京医科大学第一附属医院老年医学科,江苏 南京 210029 
王 龙 南京医科大学第一附属医院老年医学科,江苏 南京 210029 
胡 淼 南京医科大学第一附属医院老年医学科,江苏 南京 210029 
丁国宪 南京医科大学第一附属医院老年医学科,江苏 南京 210029 
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中文摘要:
      目的:探索小鼠棕色脂肪细胞原代培养的方法?方法:取C57BL/6J小鼠棕色脂肪组织,采用胶原酶消化过滤法获得梭形细胞,对培养出来的细胞进行形态学观察,诱导分化后用油红O染色法染色定性,荧光定量PCR检测棕色脂肪标志基因表达情况?结果:培养出的梭形细胞成分均一,增殖旺盛,诱导分化后分化率高,经油红O染色证实为脂肪细胞,荧光定量PCR检测棕色脂肪标志基因UCP-1表达量明显升高?结论:从C57BL/6J小鼠棕色脂肪组织中可以分离出具有很强增殖?分化能力的前棕色脂肪细胞,这种棕色脂肪细胞原代培养模型的建立为在体外进一步研究棕色脂肪的功能提供了良好的基础?
英文摘要:
      Objective: To establish a culture method for primary mouse brown adipocyte precursor cells. Methods: Fibroblast-like cells were collected from C57BL/6j mice brown adipose tissue. The morphological changes of the cultured cells were observed,the intracytoplasmic lipid of the culture cells was determined using oil red O staining,and the expression of the brown adipocyte specific gene was determined by real time PCR. Results: The cultured fibroblast-like cells showed highly homogeneous appearance with active proliferation and differentiate into mature adipocytes. Oil red O staining,morphological observation,and expression of brown adipocyte specific gene UCP-1 verified these cells as brown adipocyte. Conclusion: Brown adipocyte precursor cells are present in C57BL/6j mice brown adipose tissue and possess the potential to proliferate and differentiate into mature brown adipocyte. The establishment of primary culture of mouse brown adipocyte precursor cells is important for furth studies of the function of brown adipocyte in vitro.
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