文章摘要
蒋 雷,王 军,张劲松.外向钾电流在谷氨酸引起的神经元损伤中的作用[J].南京医科大学学报,2011,(12):1756~1759
外向钾电流在谷氨酸引起的神经元损伤中的作用
The involvement of outward K+ currents in glutamate-induced neuronal damage
投稿时间:2011-09-10  
DOI:10.7655
中文关键词: 外向钾电流  谷氨酸  神经元  细胞凋亡
英文关键词: outward K+ current  glutamate  neuron  cell apoptosis
基金项目:江苏省卫生厅面上项目(H201005)
作者单位
蒋 雷 南京医科大学第一附属医院急诊医学中心,江苏 南京 210029 
王 军 南京医科大学应用毒理学重点实验室,江苏 南京 210029 
张劲松 南京医科大学第一附属医院急诊医学中心,江苏 南京 210029 
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中文摘要:
      目的:探讨谷氨酸通过(NMDA)受体对外向钾电流的影响以及引起神经元损伤的作用机制?方法:以分离出的孕18 d SD大鼠胎鼠的皮层神经元作为实验对象,分为对照组和谷氨酸处理组,利用全细胞膜片钳的方法记录外向钾电流的变化,通过细胞形态学和TUNEL的方法分别观察谷氨酸处理后神经元的损伤情况?结果:谷氨酸能明显引起外向钾电流的增大,而NMDA受体抑制剂MK-801明显抑制由谷氨酸引起的外向钾电流的增大?形态学以及TUNEL实验研究表明,谷氨酸明显引起神经元的损伤(P < 0.01),而MK-801?钾通道抑制剂TEA以及高浓度的外钾溶液能明显减缓由谷氨酸引起的细胞损伤(P < 0.01)?结论:外向钾电流参与了谷氨酸引起的神经元的损伤,但其机制仍需进一步的探讨?
英文摘要:
      Objective:To explore the effects of glutamate and the NMDA receptor on the outward K+ currents,and illustrate the mechanisms for glutamate induced neuronal damage. Methods: Neurons were collected from18-day-old embryonic rats in the present work and were divided into two groups:the control and glutamate treated group. The currents were recorded by performing the whole cell patch clamp,and subsequently the cell morphous as well as neuron apoptosis were evaluated by morphology and TUNEL assay. Results: Glutamate obviously increased the outward K+ currents and MK-801,the NMDA receptor antagonist,significantly alleviated the outward K+ currents. The morphology and TUNEL results revealed that the treatment of glutamate resulted in neuronal damage (P < 0.01) while MK-801,the blocker of K+ channel TEA,as well as high concentration of K+ solution substantially reduced the cell damage(P < 0.01). Conclusion: The outward K+ currents were involved in neuronal damage induced by glutamate,and the mechanisms need further clarified.
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