文章摘要
杨 叶,乔善磊,陈景衡,茅 力.抗氧化营养素对丙烯酰胺所致雄性大鼠生殖氧化损伤的保护作用[J].南京医科大学学报,2011,(12):1764~1767
抗氧化营养素对丙烯酰胺所致雄性大鼠生殖氧化损伤的保护作用
The protection effect of antioxidant nutrients on oxidative damage of acrylamide of male reproduction in rats
投稿时间:2011-06-07  
DOI:10.7655
中文关键词: 丙烯酰胺  环氧丙酰胺  抗氧化营养素  细胞色素P450 2E1  抗氧化酶
英文关键词: acrylamide  glycidamide  antioxidant nutrients  cytochrome P450 2E1  antioxidase
基金项目:南京医科大学科技发展基金重点项目资助(08NMUZ010)
作者单位
杨 叶 南京医科大学公共卫生学院卫生检验学系,国家级预防医学实验教学示范中心,江苏 南京 210029 
乔善磊 南京医科大学公共卫生学院卫生检验学系,国家级预防医学实验教学示范中心,江苏 南京 210029 
陈景衡 南京医科大学公共卫生学院卫生检验学系,国家级预防医学实验教学示范中心,江苏 南京 210029 
茅 力 南京医科大学公共卫生学院卫生检验学系,国家级预防医学实验教学示范中心,江苏 南京 210029 
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中文摘要:
      目的:探讨某些抗氧化营养素对丙烯酰胺(acrylamide,AA)所致雄性大鼠生殖氧化损伤的保护作用,为预防AA对人体的生殖危害提供一定科学依据?方法:选择SD雄性大鼠72只随机分成阴性对照组? 阳性染毒组?VitE组?VitC组?硒组?VitE+硒组?染毒4周后处死大鼠,收集血清?肝脏?睾丸和附睾?测定血清中AA和环氧丙酰胺(glycidamide,GA)含量,血清?睾丸中丙二醛(MDA)?超氧化物歧化酶(SOD)和谷胱甘肽S转移酶(GSH-ST)的含量,肝微粒体细胞色素P450 2E1活性?结果:阳性染毒组与阴性对照组相比体重?肝脏系数和附睾系数均减小,血清SOD?GSH-ST水平和睾丸MDA水平差异显著;而抗氧化营养素各组与阳性染毒组相比血清SOD?GSH-ST水平和睾丸MDA水平差异显著;VitE+硒组睾丸MDA水平与VitE?硒组差异显著;肝微粒体细胞色素P450 2E1活性VitE?VitC组与阳性染毒组差异显著?结论:抗氧化营养素对AA所致雄性大鼠生殖氧化损伤具有一定的保护作用,VitE和硒联合使用的保护效果更好?
英文摘要:
      Objective:To evaluate antioxidant nutrients’ protection on reproductive oxidative damage male rats induced by acrylamide(AA). Methods:Six-week-old SD male rats were randomly divided into 6 groups. The six groups were negative control group,positive exposure group,VitE group,VitC group,selenium group and VitE+selenium group,respectively. After four weeks,rats were killed to collect serum,liver,testis,epididymis,and then the contents of AA,glycidamide (GA),malonaldehyde(MDA),superoxide dismutase (SOD),glutathione-S-transferase(GSH-ST) and liver microsomal cytochrome P450 2E1 were determined. Results: Rats exposed to AA had significantly decreased weight,liver index and epididymis coefficient,and the SOD,GSH-ST levels in serum and the MDA level in testis were significant different compared with the controls. Compared with the positive exposure groups,the SOD,GSH-ST levels in serum and the MDA level in testis of the antioxidant nutrients groups were significant different. Compared with the VitE,selenium groups,the MDA levels in testis of VitE+Se group had significant differences. The activity of liver microsomal cytochrome P450 2E1 in VitE and VitC groups had a statistical difference compared with the positive exposure group. Conclusion: The antioxidant nutrients had a protective effect on male reproductive oxidative damage in rats induced by AA,and VitE+selenium protective effect of better use.
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