文章摘要
姜 超,孙 正,许 哲,陈 彻,姚学权,李为苏,刘福坤.5-FU相关代谢酶表达与胃癌化疗敏感性的关系[J].南京医科大学学报,2011,(12):1815~1819
5-FU相关代谢酶表达与胃癌化疗敏感性的关系
The relationship between expression levels of 5-FU-associated metabolic enzyme genes and the sensitivity of neoadjuvant chemotherapy in gastric carcinomas
投稿时间:2011-08-29  
DOI:10.7655
中文关键词: 新辅助化疗  胸腺嘧啶合成酶  二氢嘧啶脱氢酶  胸腺嘧啶酸化酶  亚甲基四氢叶酸还原酶
英文关键词: neoadjuvant chemotherapy  thymidylate synthase  dihydropymidine dehydrogenase  thymidine phosphorylas  methyle-netetrahydrofolate reductase
基金项目:中华国际医学交流基金会抗肿瘤治疗专项科研基金(IMF-F-H00I-035)
作者单位
姜 超 江苏省中医院消化肿瘤外科,江苏 南京 210029 
孙 正 江苏省中医院消化肿瘤外科,江苏 南京 210029 
许 哲 江苏省中医院消化肿瘤外科,江苏 南京 210029 
陈 彻 江苏省中医院消化肿瘤外科,江苏 南京 210029 
姚学权 江苏省中医院消化肿瘤外科,江苏 南京 210029 
李为苏 江苏省中医院消化肿瘤外科,江苏 南京 210029 
刘福坤 江苏省中医院消化肿瘤外科,江苏 南京 210029 
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中文摘要:
      目的:比较新辅助介入化疗前后胃癌组织内胸腺嘧啶合成酶(thymidylate synthase,TS),二氢嘧啶脱氢酶(dihydropymidine dehydrogenase,DPD),胸腺嘧啶磷酸化酶(thymidine phosphorylase,TP),亚甲基四氢叶酸还原酶(methylenetetrahydrofolate reductase,MTHFR)等5-FU相关代谢酶表达,探讨其与化疗疗效的相关性?方法:对49例进展期胃癌患者随机分为新辅助化疗手术组(39例)和直接手术组(10例),对胃镜活检及手术后组织标本行实时定量PCR检测其中TS?DPD?TP和MTHFR的表达?结果:新辅助化疗组分为:化疗有效组(27)例和无效组(12例),有效率69.2%;化疗前有效组4种基因的表达水平与无效组相比均低,其中DPD差异较明显(P < 0.05);化疗后有效组TS?TP?DPD表达较无效组低50.3%?51.3%和31.2%(P < 0.05);有效组TS和TP较化疗前分别下降38.6%和48.7%(P < 0.05),而无效组则前后变化不明显(P > 0.05)?结论:介入化疗前肿瘤内DPD表达或者介入后TS?TP?DPD表达以及介入前后TS和TP表达变化程度与化疗敏感性相关?
英文摘要:
      Objective: To compare the pre- and post-neoadjuvant intervention chemotherapeutic expression levels of thymidylate synthase(TS),dihydropymidine dehydrogenase(DPD),thymidine phosphorylase(TP),and methylenetetrahydrofolate reductase (MTHFR),and to explore the relationship between the expression of these four genes and the neoadjuvant intervention chemotherapeutic effects. Methods: Forty-nigh patients with advanced gastric cancer were divided into neoadjuvant chemotherapeutic group (39 patients) and operative group (10 patients). The gene expression levels of TS,TP,DPD,and MTHFR in biopsy specimens from gastroscope and tumor tissue specimens after surgical resection were detected by real-time PCR. Results: According to the results of histopathology,neoadjuvant chemotherapeutic patients were divided into chemotherapeutic effective group (27 cases) and noneffective group (12 cases). The effective rate of neoadjuvant chemotherapy was 69.2%. The expression levels of four detected genes in the tumors of effective patients before neoadjuvant intervention chemotherapy were generally lower than that of the noneffective cases,and the level of DPD decreased 51.2%(P < 0.05). After neoadjuvant intervention chemotherapy,the levels of TS,TP and DPD in the effective group were 50.3%,51.3%,and 31.2% lower than those of noneffective group,respectively (all P < 0.05). The expression levels of TS and TP in effective group decreased by 38.6% and 48.7% compared with that before chemotherapy,respectively (P < 0.05),while the levels of four genes in noneffective group exhibited no significant difference between before and after chemotherapy (P > 0.05). Conclusion: The patients with low level of DPD expression in tumors before neoadjuvant chemotherapy and low levels of TS,TP,DPD after neoadjuvant chemotherapy were sensitive to the neoadjuvant intervention chemotherapy. And the decreased levels of TS and TP after neoadjuvant chemotherapy were important for evaluating the prognosis of neoadjuvant chemotherapy.
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