文章摘要
许佩文,陆小伟,朱陵君,陈 刚.顺铂纳米微球治疗骨肉瘤效果评价[J].南京医科大学学报,2011,(12):1820~1822
顺铂纳米微球治疗骨肉瘤效果评价
The evaluation on cisplatin-loaded micelles against osteosarcoma in vitro
投稿时间:2011-09-07  
DOI:10.7655
中文关键词: 顺铂  纳米微球  骨肉瘤
英文关键词: cisplatin  micelles  osteosarcoma
基金项目:江苏省教育厅自然科学基金资助(10KJB320008)
作者单位
许佩文 南京医科大学第一附属医院老年医学科,江苏 南京 210029 
陆小伟 南京医科大学第一附属医院老年医学科,江苏 南京 210029 
朱陵君 南京医科大学第一附属医院肿瘤科,江苏 南京 210029 
陈 刚 南京医科大学附属南京市江宁医院肿瘤外科,江苏 南京 211000 
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中文摘要:
      目的:制备顺铂载药纳米微球,并通过理化手段检测其各项特征,考察其体外对骨肉瘤细胞的杀伤效果?方法:合成PCL-PEG二嵌段共聚物,采用溶剂分散法制备顺铂载药纳米微球?测定该顺铂载药微球对人骨肉瘤U-20s细胞系的杀伤效果?结果:所制备的载药微球粒径< 100 nm?载药量在5%左右,包封率则超过80%?体外稳定性实验证实了顺铂微球在室温下具有良好的稳定性?体外释放曲线显示了顺铂微球良好的缓释特性?MTT实验显示顺铂载药微球同样具有浓度依赖性的细胞杀伤效果,其各个时间点IC50值均稍大于顺铂裸药?结论:顺铂载药纳米微球具有一定的抗骨肉瘤作用,该种顺铂纳米给药系统作为一种新型的抗骨肉瘤纳米制剂值得进一步研究?
英文摘要:
      Objective: To explore the in vitro antitumor effect of cisplatin-loaded micelles on osteosarcoma cell line U-20 s. Methods: Cisplatin-loaded micelles were prepared by a nano-precipitation method with poly (ethylene glycol)-polycaprolactone (mPEG-PCL) as drug carrier. In vitro cytotoxicity of cisplatin-loaded micelles against osteosarcoma cell line U-20s was assessed by MTT assay. Results: The size of cisplatin-loaded micelles is less than 100 nm. The drug loading content is nearly 5% with the encapsulation efficiency more than 18%. In vitro stability test shows cisplatin-londed micelles are stable at routime time. In vitro release study indicates that cisplatin can be released from the core-shell structure of polymeric micelles in a sustained manner. In vitro cytotoxicity assessment shows that free cisplatin and cisplatin-loaded micelles display similar time and dose dependent cytotoxicity against osteosarcoma cell line U-20s. The IC50 value of cisplatin-loaded micelles is bigger than that of free cisplatin at each corresponding time. Conclusion: In vitro evaluation of cisplatin-loaded micelles demonstrates effective antitumor effects compared with free drugs. Therefore,development of nanosized drug delivery systems emerges as a novel field in the research of anticancer drugs,which merits more intensive studies and reveals potential application.
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