文章摘要
孔宏亮,宋丽杰,李占全,袁 龙.人参皂甙Rbl对阿霉素心力衰竭大鼠致心脏纤维化因子表达的影响[J].南京医科大学学报,2012,(1):26~29
人参皂甙Rbl对阿霉素心力衰竭大鼠致心脏纤维化因子表达的影响
The effects of Ginsenoside-Rbl on myocardial fibrosis related protein and mRNA in rats with adriamycin-induced chronic heart failure
投稿时间:2011-09-22  
DOI:10.7655
中文关键词: 人参皂甙Rbl  心肌重构  转化生长因子-β1  结缔组织生长因子  内皮素-1
英文关键词: Ginsenosides-Rbl  chronic heart failure  transforming growth factor β1  connective tissue growth factor  endothelin 1
基金项目:辽宁省自然科学基金资助项目(201102107)
作者单位
孔宏亮 辽宁省人民医院心脏中心,辽宁 沈阳 110016 
宋丽杰 辽宁省人民医院心脏中心,辽宁 沈阳 110016 
李占全 辽宁省人民医院心脏中心,辽宁 沈阳 110016 
袁 龙 辽宁省人民医院心脏中心,辽宁 沈阳 110016 
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中文摘要:
      目的:探讨人参皂甙Rbl(Ginsenosides-Rbl,Gs-Rb1)是否通过心脏抑制心脏纤维化相关蛋白和mRNA介导其改善慢性心力衰竭(chronic heart failure,CHF)大鼠的心肌重构效应?方法:在构建阿霉素CHF大鼠模型成功后,将其随机分为Gs-Rb1组(n = 17)给予Gs-Rb1 70 mg/(kg·d)和CHF组(n = 15),另外选取同龄健康大鼠作为对照组(n = 10)?第4周心脏超声评估全心质量指数(HW/BW)和左室质量指数(LW/BW),应用Western blot和RT-PCR方法检测转化生长因子-β1 (transforming growth factor β1,TGF-β1)?结缔组织生长因子(connective tissue growth factor,CTGF)?内皮素-1(endothelin 1,ET-1)等蛋白和mRNA的表达?结果:与对照组相比,Gs-Rb1组全心质量指数(HW/BW,P = 0.005)及左室质量指数(LW/BW,P = 0.000)均显著改善;与CHF组相比,Gs-Rb1组TGF-β1?CTGF和ET-1蛋白的表达均显著下降(P均< 0.05),但均显著高于对照组(P均 < 0.05);Gs-Rb1组TGF-β1?CTGF和ET-1等mRNA的表达均显著低于CHF组(P均 < 0.05),但均显著高于对照组(P均 < 0.05)?结论:在Gs-Rbl改善CHF大鼠心肌重构效应中,Gs-Rbl可通过下调TGF-β1?CTGF?ET-1等蛋白和mRNA等介导其保护作用?
英文摘要:
      Objective:To elucidate whether Ginsenosides-Rbl (Gs-Rb1) improved myocardial remodeling and whether Gs-Rb1 improved myocardial fibrosis related protein/mRNA in rat heart with chronic heart failure(CHF) induced by adriamycin. Methods:CHF models of rats were performed by adriamycin. CHF rats were randomly divided into CHF group (n = 15) and Gs-Rb1 group(n = 17) that received 70 mg/(kg·d) Gs-Rb1. In addition,age-matched health rats were referred as control group(n = 10). At the 4th week,the heart to body weight (HW/BW) and the left ventricular to body weight (LW/BW) was analyzed by echocardiography. Histology was used to assess myocardial interstitial fibrosis in the heart. The expression of transforming growth factor β1 (TGF-β1),connective tissue growth factor (CTGF) and endothelin 1 (ET-1) were detected by Western blot and RT-PCR. Results: Compared to CHF group,Gs-Rb1 significantly improved HW/BW(P = 0.005) and LW/BW(P = 0.000);The protein expression of TGF-β1,CTGF and ET-1 were more significantly down-regulated in Gs-Rb1 group than in CHF group,which in those both group were significantly higher than those in control group(P < 0.05);The mRNA expression of TGF-β1,CTGF and ET-1 were more significantly down-regulated in Gs-Rb1 group than in CHF group,which in the both group were significantly higher than in control group(P < 0.05). Conclusion:The effects of Gs-Rb1,on improving myocardial remodeling in CHF rats,were fulfilled by increasing protein and mRNA of TGF-β1,CTGF and ET-1.
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