文章摘要
袁 俐,姜声扬,虞 珏,任亚丽,徐文平,孙 明,德 伟.雷米普利对2型糖尿病大鼠microRNA表达谱的影响[J].南京医科大学学报,2012,(1):45~49
雷米普利对2型糖尿病大鼠microRNA表达谱的影响
Expression changes of microRNAs in 2 type diabetes’ rats treated with ramipril
投稿时间:2011-06-29  
DOI:10.7655
中文关键词: 2型糖尿病  雷米普利  miRNA
英文关键词: 2 type diabetes  ramipril  miRNA
基金项目:南通市科技局基金资助(K2009017)
作者单位
袁 俐 南通体臣卫生学校内科学教研室,江苏 南通 226007 
姜声扬 南通体臣卫生学校内科学教研室,江苏 南通 226007 
虞 珏 南通体臣卫生学校内科学教研室,江苏 南通 226007 
任亚丽 南通体臣卫生学校内科学教研室,江苏 南通 226007 
徐文平 南通体臣卫生学校内科学教研室,江苏 南通 226007 
孙 明 南京医科大学分子生物教研室,江苏 南京 210029 
德 伟 南京医科大学分子生物教研室,江苏 南京 210029 
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中文摘要:
      目的:建立链脲霉素(streptozocin,STZ)+高糖高脂饮食诱导2型糖尿病大鼠模型,并以血管紧张素转化酶抑制剂(angiotensin-converting enzyme inhibitor,ACEI)药物雷米普利处理,筛查用雷米普利后2型糖尿病大鼠胰腺组织microRNA(miRNA)表达的变化,并进行实时定量PCR验证芯片结果,寻找与2型糖尿病功能改善相关的miRNA?方法:STZ(40 mg/kg)腹腔注射+高脂高糖饲料喂饲健康雄性SD大鼠,测随机血糖≥16.7 mmol/L成模后1周,雷米普利50 mg/(kg·d)灌胃28 d?实验结束后,取胰腺组织常规抽提总RNA,miRNA芯片检测miRNA表达谱的改变?结果:2型糖尿病大鼠经雷米普利处理后,miRNA表达谱中出现明显差异表达(1.5倍以上)的miRNA共有27个,其中20个表达上调,7个表达下调?结论:在2型糖尿病大鼠模型中,用雷米普利处理后部分胰腺miRNA的表达发生明显变化,提示miR-1?miR-30c可能参与胰腺功能改善的调节?
英文摘要:
      Objective: To investigate the expression pattern of microRNA(miRNA) in pancreas of 2 type diabetes rats treated with ramipril by miRNA array technique. Methods: Male Spmgue-Dawley (SD) rats were injected in peritoneal with streptozocin (40 mg/kg) and fed with high sugar and fat for 1 weeks,then treated with ramipril [50 mg/(kg·d)] for 28 days. When the rats were sacrificed,total RNA was extracted from myocardium and the differential expressions of miRNA were detected by miRNA array. Results: Contrast to control group rats,the expression level of 27 miRNAs changed significantly in ramipril group rats,20 of them was up-regulated and the other 7 were down-regulated. Conclusion: The expression profile of miRNA changes in pancreas of 2 type diabetes rats treated with ramipril,which implies that those changed miRNAs may play an important role in improvement of pancreas function in SD rats.
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