泛耐药鲍氏不动杆菌获得性耐药相关基因与可移动遗传元件检测指标的聚类分析
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无锡市医院管理中心科技发展基金项目(YGM1001)


Index cluster analysis of acquired resistance-related genes and mobile genetic elements in pandrug-resistant Acinetobacter baumannii
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    目的:调查泛耐药鲍氏不动杆菌(pandrug-resistant Acinetobacter baumannii,PDR-ABA)中获得性耐药相关基因和可移动遗传元件遗传标记的存在状况,以及两者的相关性-方法:收集2010年3月到2010年5月临床标本中分离的PDR-ABA菌20株,采用聚合酶链反应(PCR)的方法检测37种β-内酰胺类获得性耐药基因-15种氨基糖苷类获得性耐药基因-5种喹诺酮类获得性耐药基因和8种可移动遗传元件,并用指标聚类分析(SPSS法)分析获得性耐药相关基因和可移动遗传元件遗传标记的相关性-结果:20株PDR-ABA菌共检出5种β-酰胺类获得性耐药基因-4种氨基糖苷类获得性耐药基因-1种喹诺酮类获得性耐药基因-5种可移动遗传元件-指标聚类分析显示获得性耐药基因与多种可移动遗传元件相关联:TEM-1-ADC-like-OXA-23-aac(6′)-Ⅰb-ant(2")-Ⅰ-ant(3")-Ⅰ-aphA1-adeB与tnpU-ISCR1-IS26-ISaba1高度关联,DHA-1-CARB与IS903关联-结论:本组PDR-ABA携带获得性耐药相关基因导致对相关药物耐药,且获得性耐药相关基因和可移动遗传元件密切相关-

    Abstract:

    Objective: To investigate the distribution of acquired resistance-related genes and markers of mobile genetic elements,and their relationships in pandrug-resistant Acinetobacter baumannii(PDR-ABA). Methods: From March 2010 to May 2010,20 strains of PDR-ABA were collected from clinical specimen. Then,57 kinds of acquired resistance-related genes against beta-lactams,aminoglycosides,quinolones,and 8 kinds of genetic markers of mobile genetic element were analyzed by PCR. The index cluster analysis was used to investigate their relationships. Results: In 20 strains of PDR-ABA,5 kinds of acquired beta-lactam-resistance genes,4 kinds of acquired aminoglycoside-resistance genes,1 kinds of acquired quinolone-resistance genes,and 5 kinds of genetic markers of mobile genetic elements were detected. Index cluster analysis showed that acquired resistance-related genes were associated with mobile genetic elements. TEM-1,ADC-like,OXA-23,aac(6′)-Ⅰb,ant(2")-Ⅰ,ant(3")-Ⅰ,aphA1 and adeB had close relations with tnpU,ISCR1,IS26,ISaba1;DHA-1 and CARB also had relations with IS903. Conclusion: In this group of PDR-ABA,acquired resistance-related genes played an important role in resistant phenotypes of antimicrobial agents,and acquired resistance-related genes were associated with mobile genetic elements.

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王春新,耿先龙,许亚丰,陈国千,赵 琪,周丽珍,糜祖煌,金 辉.泛耐药鲍氏不动杆菌获得性耐药相关基因与可移动遗传元件检测指标的聚类分析[J].南京医科大学学报(自然科学版),2012,(1):115-118

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  • 收稿日期:2011-09-26
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