文章摘要
王春新,耿先龙,许亚丰,陈国千,赵 琪,周丽珍,糜祖煌,金 辉.泛耐药鲍氏不动杆菌获得性耐药相关基因与可移动遗传元件检测指标的聚类分析[J].南京医科大学学报,2012,(1):115~118
泛耐药鲍氏不动杆菌获得性耐药相关基因与可移动遗传元件检测指标的聚类分析
Index cluster analysis of acquired resistance-related genes and mobile genetic elements in pandrug-resistant Acinetobacter baumannii
投稿时间:2011-09-26  
DOI:10.7655
中文关键词: 鲍氏不动杆菌  获得性耐药基因  可移动遗传元件  指标聚类分析  泛耐药
英文关键词: Acinetobacter baumannii  acquired resistance gene  mobile genetic elements  index cluster analysis  pandrug-resistant
基金项目:无锡市医院管理中心科技发展基金项目(YGM1001)
作者单位
王春新 南京医科大学附属无锡人民医院医学检验科,江苏 无锡 214023 
耿先龙 南京医科大学附属无锡人民医院医学检验科,江苏 无锡 214023 
许亚丰 南京医科大学附属无锡人民医院医学检验科,江苏 无锡 214023 
陈国千 南京医科大学附属无锡人民医院医学检验科,江苏 无锡 214023 
赵 琪 南京医科大学附属无锡人民医院医学检验科,江苏 无锡 214023 
周丽珍 南京医科大学附属无锡人民医院医学检验科,江苏 无锡 214023 
糜祖煌 无锡市克隆遗传技术研究所,江苏 无锡 214026 
金 辉 东南大学公共卫生学院流行病与卫生统计教研室,江苏 南京 210096 
摘要点击次数: 956
全文下载次数: 810
中文摘要:
      目的:调查泛耐药鲍氏不动杆菌(pandrug-resistant Acinetobacter baumannii,PDR-ABA)中获得性耐药相关基因和可移动遗传元件遗传标记的存在状况,以及两者的相关性?方法:收集2010年3月到2010年5月临床标本中分离的PDR-ABA菌20株,采用聚合酶链反应(PCR)的方法检测37种β-内酰胺类获得性耐药基因?15种氨基糖苷类获得性耐药基因?5种喹诺酮类获得性耐药基因和8种可移动遗传元件,并用指标聚类分析(SPSS法)分析获得性耐药相关基因和可移动遗传元件遗传标记的相关性?结果:20株PDR-ABA菌共检出5种β-酰胺类获得性耐药基因?4种氨基糖苷类获得性耐药基因?1种喹诺酮类获得性耐药基因?5种可移动遗传元件?指标聚类分析显示获得性耐药基因与多种可移动遗传元件相关联:TEM-1?ADC-like?OXA-23?aac(6′)-Ⅰb?ant(2")-Ⅰ?ant(3")-Ⅰ?aphA1?adeB与tnpU?ISCR1?IS26?ISaba1高度关联,DHA-1?CARB与IS903关联?结论:本组PDR-ABA携带获得性耐药相关基因导致对相关药物耐药,且获得性耐药相关基因和可移动遗传元件密切相关?
英文摘要:
      Objective: To investigate the distribution of acquired resistance-related genes and markers of mobile genetic elements,and their relationships in pandrug-resistant Acinetobacter baumannii(PDR-ABA). Methods: From March 2010 to May 2010,20 strains of PDR-ABA were collected from clinical specimen. Then,57 kinds of acquired resistance-related genes against beta-lactams,aminoglycosides,quinolones,and 8 kinds of genetic markers of mobile genetic element were analyzed by PCR. The index cluster analysis was used to investigate their relationships. Results: In 20 strains of PDR-ABA,5 kinds of acquired beta-lactam-resistance genes,4 kinds of acquired aminoglycoside-resistance genes,1 kinds of acquired quinolone-resistance genes,and 5 kinds of genetic markers of mobile genetic elements were detected. Index cluster analysis showed that acquired resistance-related genes were associated with mobile genetic elements. TEM-1,ADC-like,OXA-23,aac(6′)-Ⅰb,ant(2")-Ⅰ,ant(3")-Ⅰ,aphA1 and adeB had close relations with tnpU,ISCR1,IS26,ISaba1;DHA-1 and CARB also had relations with IS903. Conclusion: In this group of PDR-ABA,acquired resistance-related genes played an important role in resistant phenotypes of antimicrobial agents,and acquired resistance-related genes were associated with mobile genetic elements.
查看全文   查看/发表评论  下载PDF阅读器
关闭