Objective:To compare the effect of three adhesive resin cements on microleakage of amalgam restoration. Methods:Forty premolars were randomly divided into four groups. The three experimental groups were restored with bonded amalgam restoration using three adhesive resin cements, respectively(Clearfil SA Luting,Panavia F,Rely X Unicem). The controls was restored with amalgam only. The teeth were immersed in 2% methylene blue solution for 4 hours and then cutted perpendicularly to the bonding surface. The interfaces were then observed under the stereomicroscope,and the depth of immertion of the methylene blue into the interface was recorded and analyzed,and finally investigate the micromorphology of the interface using the scanning electron microscopy. Results:The microleakage of the three experimental groups is less than that of the control group. And there is no differences among the three experimental groups. The microleakage at gingival margin is significantly greater than that of the occlusal margin. Conclusion:The adhesive resin cement can significantly improve the marginal seal of amalgam restorations.