Objective: To evaluate the capacity of of three cements on polymorph nuclear neutrophils. Methods: The bottom of 24-well plates were coved by glass ionomer cement,zinc polycarboxylate cement,and light-cured resin cement respectively. Polymorph nuclear neutrophils(PMNs)were isolated from blood collected from healthy volunteer donors. PMNs were co-incubated with or without cement for 4 hours. Flow cytometry was used to detect cell viability and reactive oxygen species(ROS) in PMNs. Concentration of interleukin-8(IL-8) in supernatant fluid was detected by ELISA. Results: The early apoptosis and necrosis rate of the control group is significantly lower,while the survival rate is significantly higher than that of cement groups. For the glass ionomer group has the highest survival rate PMNs is and the lowest rates of the early apoptosis and the necrosis. For the glass iron group,the ROS level of PMNs demonstrates a significantly increase. The concentrations of IL-8 of the cement groups are significantly higher than that of the control group,and the glass ionomer cement group has the highest level,while the zinc polycarboxylate cement group has the lowest. Conclusion: The glass ionomer displays characteristics of relative excellent antibacterial activities and lower inflammatory activation.