Objective:To investigate the expression of RhoA and ROCK2 in cervical intraepithelial neoplasia(CIN) and cervical cancer and its correlation with invasiveness. Methods: The expressions of RhoA and ROCK2 were examined respectively by immunohistochemical S-P method in 10 cases of cervicitis tissue,30 cases of cervical intraepithelial neoplasia and 30 cases of cervical cancer. The correlation between the immunohistochemical results and the clinicopathological features were analyzed. Results: ① The expression rate of RhoA and ROCK2 were 0% in cervicitis tissues,36.7% and 30.0% in cervical intraepithelial neoplasia,83.3% and 76.7% in cervical cancer,respectively. The positive rate was significantly higher in the cervical cancer group than in the normal control group(P < 0.01). From cervicitis tissue to cervical intraepithelial neoplasia and to cervical cancer,the positive expression rates of RhoA and ROCK2 were gradually increased. ② RhoA and ROCK2 protein expression were correlated with histological grade and lymph node metastasis(P < 0.05),but not with age and clinical stage (P > 0.05). Conclusion: Expression of RhoA and ROCK2 may serve as a parameter for evaluating metastasisand prognosis in cervical cancer.