文章摘要
连一新,施敏骅.缺氧诱导因子-1α和环氧化物酶-2在肺癌组织中的表达及其与血管生成的关系[J].南京医科大学学报,2012,(5):690~694
缺氧诱导因子-1α和环氧化物酶-2在肺癌组织中的表达及其与血管生成的关系
The expression of hypoxia-inducible factor 1alpha (HIF-1α) and cyclooxygenase 2 (COX-2) and the correlation with angiogenesis in human lung cancer
投稿时间:2012-03-01  
DOI:10.7655
中文关键词: 肺癌  缺氧诱导因子-1α  环氧化物酶-2  微血管密  血管形成
英文关键词: lung cancer  angiogenesis  cyclooxygenase-2  microvessel density  hypoxia-inducible factor 1alpha
基金项目:苏州大学青年教师科研基金(Q3123640)
作者单位
连一新 苏州大学附属第二医院呼吸科,江苏 苏州 215004 
施敏骅 苏州大学附属第二医院呼吸科,江苏 苏州 215004 
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中文摘要:
      目的:探讨肺癌组织中缺氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)?环氧化物酶-2(cyclooxygenase 2,COX-2)的表达及其与病灶部位微血管密度(microvessel density,MVD)?肺癌临床分期和病理分型的相互关系?方法:免疫组化法检测48例肺癌组织中HIF-1α?COX-2的表达,用CD34单克隆抗体标记血管内皮细胞并计数MVD?结果:48例肺癌组织中HIF-1α的阳性表达率为87.50%(42/48),HIF-1α的表达与组织病理分型无关(P > 0.05)?HIF-1α在早期(Ⅰb~Ⅲa期)肺癌组的阳性表达率为78.95%(15/19),在晚期(Ⅲb~Ⅳ期)肺癌组为93.10%(27/29),两组差异有统计学意义(P < 0.05)?48例肺癌组织中COX-2阳性表达率为77.08%(37/48),COX-2的表达与组织病理分型无关(P > 0.05)?早期肺癌组COX-2的阳性表达率为57.89%(11/19),晚期为89.66%(26/29),两组差异有统计学意义(P < 0.05)?肺癌组织中HIF-1α?COX-2 的表达呈正相关(P < 0.05)?随着HIF-1α或COX-2表达强度的增加,病灶部位MVD值增高(P < 0.05 )?MVD在HIF-1α?COX-2均为阳性表达的组织中的值显著高于其在两者均为阴性表达的组织中的值或其中任一因素为阳性表达的组织中的值(P < 0.05)?结论:HIF-1α和COX-2在肺癌及其血管形成过程中起着重要作用,并且两者间可能存在一定的协同关系?
英文摘要:
      Objective: To examine the expression of hypoxia-inducible factor 1alpha (HIF-1α),cyclooxygenase 2 (COX-2) and the correlation with microvessel density(MVD) in human lung cancer. Methods: HIF-1-α and COX-2 expression levels were determined by immunohistochemistry in lung cancer tissues of 48 patients. MVD was determined by immunostaining with anti-CD34 antibody. Results: HIF-1α and COX-2 were positive in 87.50%(42/48) and 77.08%(37/48),respectively,in 48 lung cancer patients. No statistical difference was found among different histopathological groups(P > 0.05). HIF-1α and COX-2 were expressed in 78.95%(15/19) and 57.89%(11/19) in early stage of lung cancer,and 93.10%(27/29) and 89.66%(26/29) in advanced stage of lung cancer. Both expressions of HIF-1 and COX-2 were positively correlated with the clinical stages of the patients(P < 0.05). In addition,the expression of HIF-1α and COX-2 were positively correlated with MVD in the lung cancer tissue(P < 0.05). The MVD of lung cancer tissue in which both HIF-1α and COX-2 were positive stained was higher than that in the group in which both HIF-1α and COX-2 or either of them was negatively expressed (P < 0.05). Conclusion: HIF-1α and COX-2 are important factors in the angiogenesis of lung cancer and may have a synergistic effect.
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