Graves病母鼠分娩后甲状腺自身抗体水平的观察
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江苏省高校自然科学研究计划第二批项目(07KJD320144)


Autoantibodies in post-partum mice with Graves’ disease
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    目的:建立Graves病小鼠模型,研究Graves病母鼠分娩后甲状腺自身抗体水平变化-方法:应用表达促甲状腺激素刺激激素受体(thyrotropic-stimulating hormone receptor,TSHR) A亚单位的重组腺病毒Ad-TSHR-289免疫雌性Balb/c小鼠制备Graves病动物模型-将6周Balb/c雌性小鼠随机分为实验组-对照组,分别用重组Ad-TSHR-289-Ad-β-gal经小鼠腿部肌肉注射,免疫剂量均为3.3 × 108 pfu/50 μl PBS,每隔3周免疫1次,共3次-免疫结束后,内眦静脉取血,检测血清甲状腺素(T4)-促甲状腺素受体抗体(TRAb),然后分别与Balb/c雄性小鼠配对,确定妊娠后继续饲养至分娩-分娩后1 d,处死母鼠,检测母鼠血清T4-TRAb-抗甲状腺过氧化物酶抗体(TPOAb)-甲状腺球蛋白抗体(TGAb),并分离母鼠甲状腺,行组织学检查-结果:实验组中96.7%的母鼠血清TRAb水平显著增高(P < 0.05);实验组第3次免疫后有56.7%(17只) 的小鼠达到甲亢标准,分娩后73.3%(22只)的母鼠达到甲亢标准;实验组中16.7%的母鼠血清TPOAb水平明显高于正常范围;母鼠血清TGAb较对照组显著增高,差异有统计学意义(P < 0.05)-结论:利用Ad-TSHR-289免疫Balb/c小鼠可以成功制备Graves病小鼠模型-分娩后Graves病母鼠血清TPOAb及TGAb水平升高可能与TRAb有关-

    Abstract:

    Objective:To illustrate the influence of "autoantibodies" in a post-partum period of a Graves’ disease animal model.Methods:Female Balb/c mice aged 6 weeks were randomly divided into experimental group and control group,and injected intramuscularly with Ad-TSHR-289(3.3 × 108 pfu/50 μl PBS) and control adenovirus(Ad-β-gal 3.3 × 108 pfu/50 μl PBS) respectively,three times at 3 weeks intervals.When the immunization was over,both groups were mated with male mice after getting a bit of serum from venae angularis for measurement of TRAb titer and T4 level. The mice were sacrificed 1 day after delivery,blood and thyroids were obtained. T4 level was detected by radio immunoassay. Levels of TRAb,TPOAb,TGAb were detected by electrochemiluminescence immunoassay. The thyroids were used for histological examination. Results:TRAb positive rates were 96.7% in experiment group(P < 0.05). The incidence of hyperthyroidism in mice after delivery was 73.3%,which was higher than that after the 3rd immunization (56.7%). TPOAb positive rates were 16.7% in experiment group. The titre of TGAb in experiment group increased siginificantly higher than that in control group(P < 0.05). Conclusion:An animal model of Graves’ disease is successfully prepared by immunization with Ad-TSHR-289. The TRAb maybe involved in the increase of TPOAb and TGAb in post-partum mice with Graves’ disease.

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余 月,王 曙,石 星,朱子阳,王 旭,郭 梅,刘倩琦. Graves病母鼠分娩后甲状腺自身抗体水平的观察[J].南京医科大学学报(自然科学版),2012,(7):928-932

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  • 收稿日期:2012-01-04
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