MCT1、CD147、CD44在非小细胞肺癌中表达的相关性及意义
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云南省卫生厅科技内设研究机构项目(2011 WS0005)


Relevance and significance of expressions of MCT1,CD147 and CD44 in non-small cell lung cancer
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    摘要:

    目的:探讨单羧酸转运蛋白1(monocarboxylate transporters 1,MCT1)-CD147-CD44 mRNA和蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)中表达的相关性及其在NSCLC发生-发展及预后中的意义-方法:取经病理确诊的56例NSCLC患者癌组织-癌旁组织(距肿瘤边缘> 5 cm)和21例肺良性病变组织采用免疫组化和实时荧光定量PCR检测MCT1-CD147-CD44蛋白及mRNA的表达-结果:①在NSCLC组织中MCT1-CD147-CD44表达均明显高于对照组(P < 0.05);②MCT1在肺癌细胞膜上的表达水平与CD147-CD44的表达均存在相关性(P < 0.05);③MCT1-CD147-CD44的表达与NSCLC患者的性别-年龄-肿瘤大小-TNM分期均无关,但与肿瘤的组织类型(P < 0.05)-淋巴转移(P < 0.05)显著相关,且MCT1-CD44蛋白和mRNA的表达与肿瘤的分化程度显著相关(P < 0.05)-结论:MCT1-CD147-CD44可能参与肿瘤的发生-发展及远处淋巴结转移,可作为检测NSCLC和预后评价的指标-

    Abstract:

    Objective:To investigate the protein and mRNA expression of monocarboxylate transporters 1(MCT1),CD147 and CD44 in non-small cell lung cancer(NSCLC),and significance of their clinical pathology. Methods:Fifty-six specimens of the NSCLC and peritumoral tissues and twenty-one control specimens of pulmonary benign diseases were studied. The mRNA and protein expressions of MCT1,CD147 and CD44 in specimens were analyzed by real-time quantitative reverse transcriptase polymerase chain reaction(real-time quantitative RT-PCR) and immunohistochemistry. Results:①The positive expression rates of MCT1,CD147 and CD44 were significantly higher in NSCLC tissues than those in the peritumoral and control tissues(P < 0.05);② The MCT1 expression in the cell membrane was correlated with both CD147 and CD44 expressions(P < 0.05);③ The expressions of MCT1,CD147 and CD44 in NSCLC were not correlated with age,gender,lesion dimension and TNM stage,but significantly correlated with histological types of tumor and lymph node metastasis (P < 0.05). And the mRNA and protein expressions of MCT1 and CD44 in NSCLC was correlated significantly with tumor differentiation degree (P < 0.05). Conclusion: MCT1,CD147 and CD44 may participate in the occurrence and development of NSCLC,and may be act as the diagnose and prognosis index of NSCLC.

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王 平,朱超男,彭 浩,周建宏,杨 慧. MCT1、CD147、CD44在非小细胞肺癌中表达的相关性及意义[J].南京医科大学学报(自然科学版),2012,(7):976-982

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  • 收稿日期:2012-03-29
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