文章摘要
赵 娜,周梅恺,叶玉伟,陈兆峰,李 强,周永宁.胃癌组织中Notch1、Jagged1及NF-κB的表达及临床意义[J].南京医科大学学报,2012,(8):1061~1065
胃癌组织中Notch1、Jagged1及NF-κB的表达及临床意义
Expression and significance of Notch1,Jagged1 and NF-κB in human gastric carcinoma
投稿时间:2011-11-23  
DOI:10.7655
中文关键词: 胃癌  Notch1  Jagged1  NF-κB
英文关键词: gastric carcinoma  Notch1  Jagged1  NF-κB
基金项目:国家自然科学基金(81172366);甘肃省技术研究与开发专项(0912027TCYP)
作者单位
赵 娜 兰州大学第一医院消化科,甘肃省胃肠病重点实验室,甘肃 兰州 730000 
周梅恺 兰州大学第一医院消化科,甘肃省胃肠病重点实验室,甘肃 兰州 730000 
叶玉伟 兰州大学第一医院消化科,甘肃省胃肠病重点实验室,甘肃 兰州 730000 
陈兆峰 兰州大学第一医院消化科,甘肃省胃肠病重点实验室,甘肃 兰州 730000 
李 强 兰州大学第一医院消化科,甘肃省胃肠病重点实验室,甘肃 兰州 730000 
周永宁 兰州大学第一医院消化科,甘肃省胃肠病重点实验室,甘肃 兰州 730000 
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中文摘要:
      目的:研究Notch1?Jagged1和NF-κB在胃癌组织中的表达状况,探讨其与临床病理特征及幽门螺旋杆菌(Helicobacter pylori,Hp)感染的关系?方法:采用组织芯片技术和免疫组化法检测60例胃癌?60例癌旁及20例正常胃黏膜组织中Notch1?Jagged1和NF-κB的表达,用Warthin-Starry银染法检测各组织中Hp的感染状况?结果:Notch1和Jagged1在胃癌组织中的阳性表达率(40.0%?70.0%)显著低于其癌旁(81.7%?96.7%)和正常组织(85.0%?100.0%),差异均有统计学意义(P < 0.05)?NF-κB在胃癌组织中的阳性表达率(66.7%)显著高于其癌旁(36.7%)和正常组织(0.0%),差异均有统计学意义(P < 0.05)?Hp在胃癌?癌旁及正常胃黏膜组织中的阳性感染率分别为76.7%?93.3%和10.0%,组间比较差异均有统计学意义(P < 0.05)?Notch1?Jagged1表达及Hp感染均与胃癌的临床病理特征无关(P > 0.05),NF-κB表达与胃癌的分化程度和TNM分期有关(P < 0.05)?Notch1在胃癌组织中的表达与Jagged1呈正相关(r = 0.460,P < 0.05),与NF-κB呈负相关(r = -0.361,P < 0.05)?Notch1?Jagged1及NF-κB在胃癌组织中的表达均与Hp感染无关(r = -0.032,r = 0.155,r = 0.028,均P > 0.05)?结论:胃癌中存在Notch1?Jagged1和NF-κB的异常表达,但均与Hp感染无关,Notch1与Jagged1低表达可能诱导NF-κB高表达,进而导致胃癌的发生?
英文摘要:
      Objective:To investigate the expression of Notch1,Jagged1 and NF-κB in gastric carcinoma and their correlation with clinicopathological features and Helicobacter pylori(Hp) infection. Methods:The expression of Notch1,Jagged1 and NF-κB was detected by tissue microarray and immunohistochemistry in 60 cases of gastric carcinoma,60 cases of adjacent gastric carcinoma and 20 cases of normal gastric mucosa tissues and Hp infection was tested by Warthin-Starry silver stain. Results:The positive rates of Notch1 and Jagged1 in gastric carcinoma tissue(40.0%,70.0%) were lower than those in adjacent gastric carcinoma tissue (81.7%,96.7%) and normal gastric tissue(85.0%,100.0%),the differences were significantly(P < 0.05). The positive rate of NF-κB in gastric cancer tissue(66.7%) was higher than those in adjacent gastric carcinoma tissue(36.7%) and normal gastric tissue(0.0%),the differences were significantly(P < 0.05). The infection rates of Hp in gastric carcinoma,adjacent gastric carcinoma and normal gastric mucosa tissues were 76.7%,93.3% and 10.0%,respectively,there were significant differences among them(P < 0.05). The expression of Notch1,Jagged1 and Hp infection were not correlated with the clinicopathological features,while the expression of NF-κB was significantly correlated with tumor differentiation status and TNM stage(P < 0.005). The expression of Notch1 was positively correlated with Jagged1(r = 0.460,P < 0.05) and negatively correlated with NF-κB(r = -0.361,P < 0.05). No correlation was shown between Hp infection and expression of Notch1,Jagged1 or NF-κB in gastric carcinoma tissues(r = -0.032,r = 0.155,r = 0.028,P > 0.05). Conclusion:The expression of Notch1,Jagged1 and NF-κB in gastric carcinoma is abnormal but not associated with Hp infection. The low expression of Notch1 and Jagged1 may induce the high expression of NF-κB,which result in the occurrence of gastric carcinoma.
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