文章摘要
王正江,杨鸿梅,姚志新,徐 岷,张尤历.DNMT3a和HDAC2在胃癌组织中的表达及临床相关性分析[J].南京医科大学学报,2012,(9):1232~1236
DNMT3a和HDAC2在胃癌组织中的表达及临床相关性分析
The correlation between DNMT3a and HDAC2 expression and clinical parameters of gastric cancer
投稿时间:2012-04-23  
DOI:10.7655
中文关键词: 胃癌  DNA甲基转移酶  组蛋白去乙酰化酶  病理
英文关键词: gastric cancer  DNA methltransferase  histone deacetylase  pathology
基金项目:盐城市医学科技发展计划项目(YK2009121);镇江市社会发展科技支撑项目(SH2009012)
作者单位
王正江 盐城市第一人民医院消化科,江苏 盐城 224001 
杨鸿梅 盐城市第一人民医院消化科,江苏 盐城 224001 
姚志新 江苏大学附属医院消化科,江苏 镇江 212001 
徐 岷 江苏大学附属医院消化科,江苏 镇江 212001 
张尤历 江苏大学附属医院消化科,江苏 镇江 212001 
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中文摘要:
      目的:检测DNA甲基转移酶3a(DNA methltransferase 3a,DNMT3a)和组蛋白去乙酰化酶2(histone deacetylase 2,HDAC2)在人胃癌组织中的表达,分析其与临床特征的相关性?方法:应用Western blot方法检测30例配对人胃腺癌和癌旁组织中DNMT3a和HDAC2表达的差异;免疫组织化学方法检测90例胃腺癌患者手术切除癌组织中DNMT3a和HDAC2的表达水平,并分析其表达与临床病理学指标的关系?结果:Western blot检测结果显示癌组织中DNMT3a和HDAC2蛋白表达量明显高于癌旁组织(P < 0.01);90例胃癌标本中,免疫组化结果显示DNMT3a阳性表达率为79%(71/90),HDAC2蛋白表达阳性率为86%(77/90),癌组织中DNMT3a表达强度与肿瘤分化程度和淋巴结转移相关(P < 0.05),HDAC2表达强度与肿瘤浸润深度?病理学大体分型?TNM分期和淋巴结转移相关(P < 0.05)?DNMT3a与HDAC2在胃癌组织中的表达呈正相关(P < 0.05)?结论:DNMT3a与HDAC2是胃腺癌发生发展中的危险因素之一,一定程度上能反映胃腺癌的恶性程度,对预后的判断有一定价值,是一项有潜在价值的肿瘤相关标志物?
英文摘要:
      Objective:To investigate the correlation between DNA methltransferase 3a(DNMT3a) and histone deacetylase 2(HDAC2) expression and clinical parameters of gastric cancer. Methods:The expressions of DNMT3a and HDAC2 in 30 gastric adenocarcinoma tissues and paracancerous tissues were detected by Western blot. The expressions of DNMT3a and HDAC2 in 90 gastric adenocarcinoma tissues were detected by immunohistochemistry. The relationship between DNMT3a and HDAC2 expressions and clinicopathological features of gastric adenocarcinoma was analyzed. Results:The protein levels of DNMT3a and HDAC2 were significantly higher than those in paracancerous tissues(P < 0.01). The immunohistochemistry examination showed that the positive rates of DNMT3a and HDAC2 protein were 79%(71/90) and 86%(77/90) respectively in 90 gastric adenocarcinoma tissues,DNMT3a expression was positively correlated with the differentiation and lymph node metastasis(P < 0.05),HDAC2 expression was positively correlated with the pathologic classification,invasion degree,TNM stage and lymph node metastasis(P < 0.05). There was positive correlation between DNMT3a and DNMT3b(P < 0.05). Conclusion:DNMT3a and HDAC2 were risk factors in the development of gastric cancer and might reflect malignant degree and prognosis to a certain degree. For these reasons,they are useful markers of the tumor.
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