文章摘要
陈建敏,安 勇,吕 楠,薛晓峰,张 烨,卫积书,苗 毅.CEACAM6介导上皮间质转化增强胰腺癌细胞侵袭能力和耐药性的研究[J].南京医科大学学报,2012,(12):1675~1679
CEACAM6介导上皮间质转化增强胰腺癌细胞侵袭能力和耐药性的研究
CEACAM6 enhances the ability of invasion and chemo-resistance in pancreatic cancer cells by inducing epithelial-mesenchymal transition(EMT)
投稿时间:2012-05-27  
DOI:10.7655/NYDXBNS20121210
中文关键词: SW1990胰腺癌细胞株  CEACAM6  上皮间质转化  侵袭  耐药
英文关键词: pancreatic cancer cell line SW1990  CEACAM6  epithelial-mesenchymal transition  invasion  chemo-resistance
基金项目:国家自然科学基金资助(30972912)
作者单位
陈建敏 南京医科大学第一附属医院普外科,江苏 南京 210029 
安 勇 南京医科大学第一附属医院普外科,江苏 南京 210029 
吕 楠 南京医科大学第一附属医院普外科,江苏 南京 210029 
薛晓峰 南京医科大学第一附属医院普外科,江苏 南京 210029 
张 烨 南京医科大学第一附属医院普外科,江苏 南京 210029 
卫积书 南京医科大学第一附属医院普外科,江苏 南京 210029 
苗 毅 南京医科大学第一附属医院普外科,江苏 南京 210029 
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中文摘要:
      目的:研究CEACAM6对胰腺癌细胞株SW1990侵袭能力和耐药性的影响,并探讨上皮间质转化(EMT)在其中的作用?方法:筛选及鉴定过表达CEACAM6的稳定转染SW1990-CEACAM6细胞株,通过定量PCR和Western blot方法检测SW1990细胞株中EMT相关表型标志物的改变?通过Transwell实验检测过表达CEACAM6对SW1990细胞株侵袭的影响,MTT法检测耐药指数(RI)?结果:定量PCR及Western blot验证过表达CEACAM6细胞株SW1990-CEACAM6成功建立,其倾向于向间质细胞转化:波形蛋白(Vimentin)表达明显上调,E-钙黏蛋白(E-cadherin)表达下降?功能实验证实过表达CEACAM6具有促进SW1990胰腺癌细胞株侵袭作用,同时细胞株耐药性增强?结论:CEACAM6能够促进SW1990胰腺癌细胞株上皮间质转化,从而促进胰腺癌细胞的恶性转化?
英文摘要:
      Objective:To investigate the potential involvement of CEACAM6 in the invasion and chemo-resistance of pancreatic cancer cell SW1990 by inducing epithelial-mesenchymal transition(EMT). Methods:After establishing and screening,CEACAM6-overexpressing cell line SW1990-CEACAM6 was observed by inverted microscope,and the changes of EMT markers were determined by Q-PCR and western blot,the invasion ability was detected by transwell,the chemo-sensitivity to gemcitabine was determined by MTT assay. Results:Q-PCR and Western blot confirmed that SW1990-CEACAM6 cell line was established successfully. Overexpression of CEACAM6 made the pancreatic cell line SW1990 prone to mesenchymal phenotype,such as up-regulation of vimentin and down-regulation of E-cadherin. SW1990-CEACAM6 cell has greater activity of invasion and chemo-resistance to gemcitabine (P < 0.05) than SW1990-EGFP cell. Conclusion:CEACAM6 could promote EMT and enhance malignant potential in terms of invasion and chemo-resistance in pancreatic cancer cell.
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