文章摘要
代守前,韦永中,余利鹏.微型CT检测血清素对雌性小鼠骨皮质和骨小梁参数的影响[J].南京医科大学学报,2013,(1):48~52
微型CT检测血清素对雌性小鼠骨皮质和骨小梁参数的影响
Effects of serotonin on cortical and trabecular bone parameters of female mice detected by Micro-CT
投稿时间:2012-10-10  
DOI:10.7655/NYDXBNS20130110
中文关键词: 血清素  小鼠  骨皮质  骨小梁  微型CT
英文关键词: serotonin  mice  cortical bone  trabecular bone  Micro-CT
基金项目:江苏省卫生厅医学科技发展基金(H2005512)
作者单位
代守前 南京医科大学第一附属医院骨科,江苏 南京 210029 
韦永中 南京医科大学第一附属医院骨科,江苏 南京 210029 
余利鹏 南京医科大学第一附属医院骨科,江苏 南京 210029 
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中文摘要:
      目的:探讨不同剂量血清素对雌性小鼠微型CT检测骨皮质和骨小梁参数的影响。方法:给予2月龄雌性C57BL/6小鼠皮下注射不同剂量(0?5?10?20?50?100 mg/kg)的血清素,3个月后通过微型CT定量分析各组小鼠第5腰椎体?股骨远端干骺端和股骨中段的相关骨参数。结果:与对照组相比,10~100 mg/kg血清素处理组小鼠骨小梁数量?骨小梁体积分数和面积分数?骨小梁矿物质密度和骨矿含量明显降低,骨小梁分离度明显升高;50~100 mg/kg血清素处理的小鼠骨皮质体积分数?骨矿含量以及骨皮质厚度明显降低。结论:血清素对小鼠骨代谢具有重要的负性调节作用。
英文摘要:
      Objective:To investigate the effects of serotonin of different doses on cortical and trabecular bone parameters of female mice. Methods:Wild-type (wt)C57BL/6 female mice with 2-month-old were injected with serotonin of different doses (0,5,10,20,50 and 100 mg/kg)subcutaneously (s.c.)daily for 3 months. Micro-CT (μCT)was used to quantitatively analyze bone parameters of the fifth lumbar bodies,distal femurs and midshafts of the femurs of each group of mice. Results:Compared to the control groups mice,the 10-100 mg/kg groups had significantly reduced trabecular numbers,trabecular bone volume and surface density,and increased trabecular separation,leading to a decrease of trabecular bone mineral density and bone mineral capacity. Mice in the 50-100 mg/kg groups caused significantly decreased cortical bone mineral capacity secondary to reduced cortical thickness and cortical bone volume density. Conclusion:Serotonin has a significantly negative regulation on bone metabolism in mice.
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