Abstract:Objective:To investigate the effect of prostaglandin E2 (PGE2) on the expression of β1-integrin and its related signaling pathway in Huh-7 cells. Methods:Huh-7 cells were treated with PGE2,EP1 receptor agonist(17-phenyltrinor Prostaglandin E2,17-PT-PGE2),EP1 receptor antagonist SC19220,nuclear factor-κappaB(NF-κB)inhibitor PDTC. Western blot and immunofluorescence test were employed to detect the expression of β1-integrin and activation of NF-κB in Huh-7 cells. Results:When Huh-7 cells were treated with 5 μmol/L PGE2 for 24 h,the level of β1-integrin was increased by 129.48% (P < 0.01). EP1 receptor agonist (5 μmol/L 17-PT-PGE2) mimicked the effect of PGE2, and increased the expression of β1-integrin by 216.34%(P < 0.01). EP1 receptor antagonist SC19220(10 μmol/L) suppressed PGE2-mediated expression of β1-integrin by 34.51% (P < 0.05). In immunofluorescence assays,NF-κB translocated into the nucleus induced by 17-PT-PGE2 in Huh-7 cells. Further,Western blot assays showed that the level of Phospho-NF-κB-P65 was increased by 209.27%(P < 0.01)after treatment of 5 μmol/L 17-PT-PGE2 for 120 min. NF-κB inhibitor PDTC decreased EP1-mediated expression of β1-integrin by 63.49% (P < 0.01). Conclusion:PGE2 might up-regulate the expression level of β1-integrin through EP1 receptor in Huh-7 cells,which was partly related to the NF-κB signaling pathway.
