文章摘要
瞿伟骏.Sigma-1受体基因敲除诱发雄鼠抑郁样行为[J].南京医科大学学报,2013,(4):421~425
Sigma-1受体基因敲除诱发雄鼠抑郁样行为
Sigma-1 receptor knockout induces depression-like behaviors in male mice
投稿时间:2013-01-07  
DOI:10.7655/NYDXBNS20130401
中文关键词: sigma-1(σ1)受体  σ1受体基因敲除(σ1受体-KO)  抑郁  雌二醇(E2)
英文关键词: sigma-1 receptor (σ1R)  σ1R knockout  depression  17β-estradiol (E2)
基金项目:国家自然科学基金(81071027)
作者单位
瞿伟骏 南京医科大学基础医学院生理学系,江苏 南京 210029 
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中文摘要:
      目的:探讨sigma-1(σ1)受体对情绪行为的调节作用。方法:采用旷场试验?悬尾试验?强迫游泳试验和Y迷宫的检测方法,分析5月龄雌和雄σ1受体基因敲除(σ1受体-KO)小鼠的抑郁样行为。用放射性免疫技术检测血浆的雌二醇和睾酮水平。结果:与野生型(WT)雄鼠相比,σ1受体-KO雄鼠在旷场中央区的运动时间明显减少,在悬尾试验和强迫游泳中的不动时间显著延长,在Y迷宫Novel臂的时间减少。但是,σ1受体-KO雌鼠的雌二醇水平和σ1受体-KO雄鼠的睾酮水平与对照组相比都没有改变。结论:σ1受体-KO诱发的抑郁样行为是雄鼠特异性的,可能与雌激素的抗抑郁作用有关。
英文摘要:
      Objective:This study examined the influence of sigma-1 receptor (σ1R) knockout on affective behaviors. Methods:We employed 5-month-old σ1R knockout (σ1R-KO) mice. Depression-like behaviors were evaluated through open-field test (OFT),tail suspension test (TST),forced swimming test (FST) and Y-maze task. Results:In comparison with male wide-type (WT) mice,male σ1R-KO mice spent less time in the center field of OFT,and had longer immobility time in FST and TST. Furthermore,the male σ1R-KO mice spent less time in the novel arm than the known arm in Y-maze. By contrast,no difference in depression-like behaviors was found between female WT mice and σ1R-KO mice. In addition,the serum levels of estrogen in female KO mice and testosterone in male KO mice were not altered compared to WT mice. Conclusion:The σ1R-KO induced depression-like behaviors were male mice-specific,and maybe related with the antidepressant effect of estrogen.
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