文章摘要
冯娇娇,殷晨阳,李昕昕,徐校佩,郭  静,王学军,王 宁,袁 栎.MIA影响非洲爪蟾胚胎中胚层的形成[J].南京医科大学学报,2013,(4):426~431
MIA影响非洲爪蟾胚胎中胚层的形成
The effect of MIA on mesoderm formation during embryo development of Xenopus lavies
投稿时间:2012-06-21  
DOI:10.7655/NYDXBNS20130402
中文关键词: 黑色素瘤抑制蛋白  非洲爪蟾  胚胎发育  中胚层
英文关键词: MIA  Xenopus lavies  embryogenesis  mesoderm
基金项目:国家自然科学基金(30971680);江苏高校优势学科建设工程资助项目;江苏省“青蓝工程”(2010)
作者单位
冯娇娇 南京医科大学生化与分子生物学系,江苏 南京 210029 
殷晨阳 南京医科大学生化与分子生物学系,江苏 南京 210029 
李昕昕 南京医科大学生化与分子生物学系,江苏 南京 210029 
徐校佩 南京医科大学生化与分子生物学系,江苏 南京 210029 
郭  静 南京医科大学生化与分子生物学系,江苏 南京 210029 
王学军 南京医科大学生化与分子生物学系,江苏 南京 210029 
王 宁 南京医科大学生化与分子生物学系,江苏 南京 210029 
袁 栎 南京医科大学生化与分子生物学系,江苏 南京 210029 
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中文摘要:
      目的:探讨黑色素瘤抑制蛋白(melanoma inhibitory activity,MIA)在非洲爪蟾早期胚胎发育中的作用。方法:根据热带爪蟾MIA序列在非洲爪蟾EST数据库中找到MIA序列,利用RT-PCR扩增全长序列并克隆至pCS2+,用于测序。利用RT-PCR检测胚胎发育各阶段MIA mRNA的表达。通过显微注射特异的反义寡核苷酸morpholino(MO)敲降MIA;利用整胚原位杂交检测胚层标志性基因的表达。结果:克隆出非洲爪蟾MIA全长序列。在胚胎发育过程中MIA从受精卵开始持续表达。敲降MIA导致胚胎胚孔闭合延缓,中胚层标志基因xbra表达明显受抑,而内胚层标志性基因sox17α表达无明显改变。结论:MIA表达于非洲爪蟾胚胎发育各阶段,敲降MIA抑制中胚层的形成。
英文摘要:
      Objective:To investigate the effect of melanoma inhibitory activity (MIA) on mesoderm formation during the embryogenesis of Xenopus lavies. Methods:The MIA sequence of Xenopus lavies was verified in EST database,and then cloned into pCS2+ for sequencing. The expression of MIA was detected during embryo development by RT-PCR analysis. The micro-injection of morpholino(MO) was used to knockdown MIA. The expression of marker genes was detected during germ layer formation by whole mount in situ hybridization. Results:The MIA sequence of Xenopus lavies was precisely cloned. The expression of MIA was detected at all the stages during embryogenesis. The knockdown of MIA by MO injection caused the delayed blastopore closure,inhibited the expression of mesodermal marker gene xbra compared to normal embryos. Conclusion:MIA is expressed during embryogenesis of Xenopus lavies,and MIA could play an important role in the mesoderm formation.
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