文章摘要
刘 昊,吴 然,贾瑞鹏,朱佳庚,于 澎,赵 炎,葛余正,吴剑平,李文成.保留肾单位术后肾脏保护新策略:缺血预适应介导内皮祖细胞归巢机制[J].南京医科大学学报,2013,(4):432~437
保留肾单位术后肾脏保护新策略:缺血预适应介导内皮祖细胞归巢机制
New strategy of renal protection after nephron sparing surgery:mechanism of ischemic preconditioning mediated endothelial progenitor cells homing
投稿时间:2012-08-15  
DOI:10.7655/NYDXBNS20130403
中文关键词: 缺血预适应  内皮祖细胞  保留肾单位手术  缺血再灌注损伤
英文关键词: ischemic preconditioning  endothelial progenitor cells  nephron sparing surgery  ischaemia-reperfusion injury
基金项目:国家自然科学基金(81070597);江苏省卫生厅科教兴卫工程领军人才与创新团队科研课题(LJ201107);江苏省六大人才高峰资助项目(2011-WS-093);江苏省科教兴卫工程重点学科开放课题(KF200942)
作者单位
刘 昊 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
吴 然 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
贾瑞鹏 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
朱佳庚 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
于 澎 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
赵 炎 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
葛余正 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
吴剑平 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
李文成 南京医科大学附属南京医院泌尿外科,江苏 南京  210006 
摘要点击次数: 946
全文下载次数: 616
中文摘要:
      目的:探讨缺血预适应(IPC)介导内皮祖细胞(EPCs)归巢对保留肾单位手术(NSS)后肾功能的保护作用。方法:SD大鼠随机分为对照组(sham)?保留肾单位组(NSS)?缺血预适应 + 保留肾单位组(IPC)。术后24 h分别留取大鼠外周血?肾脏组织。采用血生化?病理学观察肾功能改变;免疫荧光观察3组大鼠肾组织中EPCs归巢情况;免疫组化技术检测肾脏微血管新生;QPCR检测血管生成因子mRNA表达。结果:IPC可显著降低大鼠血肌酐水平及肾小管损伤评分(P < 0.05),促进EPCs归巢至损伤肾脏组织。与sham组和NSS组相比,IPC组大鼠肾脏组织微血管新生显著增强(P < 0.05),且基质细胞衍生因子1(SDF-1)mRNA表达升高(P < 0.05)。结论:IPC可介导EPCs归巢至损伤肾脏,后者可能通过释放血管生成因子,促进肾脏微血管新生,达保护肾功能之目的。
英文摘要:
      Objective:To investigate the role of endothelial progenitor cells(EPCs) in modulation of ischemic injury in a nephron sparing surgery (NSS) rat model by ischemic preconditioning (IPC). Methods:Sprague-Dawley rats were randomly divided into three groups after right-side kidney nephrectomy:the Sham group underwent surgery without vascular clamping;the NSS group,the renal blood vessels were clamped for 40 min and partial nephrectomy(PN) was performed;the IPC group,the rats were pretreated with 15 min ischemia and 10 min reperfusion. At 24 h after reperfusion,the pool of circulating and kidneys were harvested. We assessed the extent of renal injury,observed the home of EPCs and detected angiogenesis as well as the expression of vascular growth factor. Results:Pretreated rats exhibited significant improvements in renal function and morphology. The number of EPCs in the kidneys was increased in the IPC groups as compared to the NSS or sham groups. Neovascularization in peritubular capillaries was markedly increased in the kidneys which treated with IPC. In addition,the expression of SDF-1 mRNA in kidneys of pretreated rats was increased compared to rats subjected to ischemic injury. Conclusion:IPC may attenuate renal ischemic injury induced by NSS;EPCs play an important role in renal protection,which involves the promotion of angiogenesis through the release of several angiogenic factors.
查看全文   查看/发表评论  下载PDF阅读器
关闭