文章摘要
陈佳佳,刘金春,常 磊,朱东亚,厉廷有.冰片及其类似物对谷氨酸诱导神经元细胞损伤的保护作用[J].南京医科大学学报,2013,(5):630~635
冰片及其类似物对谷氨酸诱导神经元细胞损伤的保护作用
Neuroprotective effect of borneol and its analogues on glutamic acid induced neuron injury
投稿时间:2012-12-14  
DOI:10.7655/NYDXBNS20130513
中文关键词: 冰片  神经保护剂  乳酸脱氢酶
英文关键词: borneol  neuroprotecting reagents  lactate dehydrogenase
基金项目:江苏省教育厅项目(ky109011108141102)
作者单位
陈佳佳 南京医科大学药学院,江苏 南京 211166 
刘金春 南京医科大学药学院,江苏 南京 211166 
常 磊 南京医科大学药学院,江苏 南京 211166 
朱东亚 南京医科大学药学院,江苏 南京 211166 
厉廷有 南京医科大学药学院,江苏 南京 211166 
摘要点击次数: 1516
全文下载次数: 1437
中文摘要:
      目的:探讨冰片及其类似物对谷氨酸诱导神经元细胞损伤的保护作用。方法:用谷氨酸刺激胎鼠原代神经元细胞,造成细胞损伤,导致乳酸脱氢酶(lactate dehydrogenase,LDH)释放到细胞外,通过测定药物抑制LDH的漏出率评价药物对神经元细胞的保护作用。结果:冰片(1)?冰片基甲酸(3)?2-冰片基乙醇(4)?2-冰片基乙酸(5)?2-羧甲基异冰片(6)?2,6-二异丙基苯甲酸(8)?2-(2’,6’-二异丙基)苯乙醇(9)?2-(2’,6’-二异丙基苯基)乙酸(10)都显示对神经元细胞的保护作用,冰片胺(2)?薄荷酸(7)则表现为神经损伤作用。结论:冰片及多数类似物对谷氨酸诱导的神经元损伤具有保护作用,其中,2,6-二异丙基苯甲酸(8)在1 × 10-5~1 × 10-7 mol/L浓度范围内都显示较好的神经保护作用,值得深入研究。
英文摘要:
      Objective:To evaluate the neuroprotective effect of borneol and its analogues on glutamic acid induced neuron damage. Methods:The lactate dehydrogenase (LDH) was leaked to extracellular space after the neurons were treated with glutamic acid. The neuroprotective effect of the test drugs was evaluated by the enzyme activity of the extracellular LDH. Results:Borneol(1),bornylfromic acid (3),2-bornylethanol (4),2-bornylacetic acid (5),2-carboxymethylisoborneol (6),2,6-diisopropylbenzonic acid(8),2-(2’,6’-diiso-propylphenyl)ethanol (9) and 2-(2’,6’-diisopropylphenyl)acetic acid (10) showed neuroprotective effect,while bornylamine (2) and menthylformic acid (7) exhibited neuron injury. Conclusion:Borneol and most of its analogues showed neuron protective effect in glutamic acid induced LDH releasing model,among which,2,6-diisopropylbenzonic acid (8) showed good neuroprotective effect in the concen-tration of 1 × 10-5~1 × 10-7 mol/L. This deserves further research.
查看全文   查看/发表评论  下载PDF阅读器