高糖腹透液对腹膜间皮细胞分泌VEGF的影响及氟伐他汀的干预作用
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金资助(81170660);江苏省科技厅基础研究计划(自然科学基金)( BK2011849);江苏省卫生厅科研基金资助(H200317)


The effect of high-glucose peritoneal dialysate and fluvastatin on the expression of vascular endothelial growth factor in human peritoneal mesothelial cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:观察氟伐他汀(fluvastatin,Flu)对高糖腹透液(high-glucose peritoneal dialysate,HGPDS)诱导的人腹膜间皮细胞(human peritoneal mesothelial cells,HPMCs)分泌血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响。方法:体外培养HPMCs,同步化48 h后,分组如下:正常对照组-HGPDS组-HGPDS+Flu组-单纯Flu组。倒置显微镜观察各组细胞形态,RT-PCR法检测各组VEGF的mRNA表达,Western blot检测各组VEGF蛋白的表达。结果:与正常对照组相比,在HGPDS作用下,48 h后细胞由铺路石样转变为长梭形,1 × 10-6 mol/L Flu可部分逆转HPMCs的形态改变。与正常对照组相比,在HGPDS作用下,人腹膜间皮细胞VEGF mRNA及蛋白表达明显增多(P < 0.05),并呈时间依赖性,VEGF mRNA和蛋白分别在HGPDS干预后12 h和48 h达高峰(P < 0.05)。Flu可明显抑制HGPDS诱导的VEGF的表达(P < 0.05),并呈浓度依赖性。结论:适当浓度的Flu可以抑制HGPDS刺激体外培养人腹膜间皮细胞VEGF表达增加。

    Abstract:

    Objective:To explore the effect of high-glucose peritoneal dialysate (HGPDS) and fluvastatin (Flu) on the expression of vascular endothelial growth factor(VEGF) in human peritoneal mesothelial cells(HPMCs). Methods:After incubated in DMEM with 0.01% FBS for 48 h, HPMCs were randomly divided as follows:control group, HGPDS group, HGPDS+Flu group, Flu alone group. The phenotype changes of HPMCs were observed by light microscopy. The mRNA expression of VEGF was observed by RT-PCR. HPMCs were treated for 48 h and the levels of VEGF protein were measured by Western blot. Results:Compared with the normal control group, after incubation with HGPDS for 48 h, the cell morphology was changed from a typical cobblestone-like appearance to fibroblast-like appearance. Flu (1 × 10-6) mol/L could partially improved the cell morphology changed by HGPDS. The mRNA and protein expressions of VEGF increased time dependently in HPMCs treated with HGPDS(P < 0.05), which peaked at 12 h and 48 h, respectively. Flu significantly inhibited the effects of HGPDS on the expression of VEGF in a dose-dependent manner(P < 0.05). Conclusion: Flu could decrease VEGF expression in HPMCs which was induced by HGPDS.

    参考文献
    相似文献
    引证文献
引用本文

武 侠,刘 佳,刘艳春,徐亚光,赵秀芬,钱 军,邢昌赢.高糖腹透液对腹膜间皮细胞分泌VEGF的影响及氟伐他汀的干预作用[J].南京医科大学学报(自然科学版),2013,(6):754-758

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2013-02-27
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2013-06-25
  • 出版日期: