顺铂诱导SPC-A1人肺腺癌细胞凋亡过程中miR-27a的表达及意义
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国家自然科学基金资助(81272324, 81000754, 81101322, 81201359);江苏省实验诊断学重点实验室基金(XK201114);江苏高校优势学科建设工程资助


Expression changes and significance of miR-27a in cisplatin-induced apoptosis of human lung adenocarcinoma cell line SPC-A1
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    摘要:

    目的:检测miR-27a在顺铂(DDP)体外诱导人肺腺癌细胞株SPC-A1凋亡过程中的表达变化,探讨其与细胞凋亡之间的相关性及其检测的临床意义。方法:体外培养SPC-A1细胞,实验组加入终浓度为2.5 μg/ml DDP,同时设置不加DDP的对照组。观察12-24-48和72 h后显微镜下细胞形态学变化,流式细胞术检测细胞凋亡率,real-time PCR检测培养上清液中和SPC-A1细胞内miR-27a的表达变化。结果:DDP(2.5 μg/ml)组SPC-A1细胞形态呈凋亡改变,在48和72 h凋亡率分别为(59.3 ± 2.5)%和(76.4 ± 3.1)%,均显著高于对照组,差异有统计学意义(P < 0.05)。DDP (2.5 μg/ml)组培养上清液中和SPC-A1细胞内miR-27a含量在24-48和72 h均显著高于对照组,差异有统计学意义(P < 0.05)。DDP (2.5 μg/ml)组培养上清液中miR-27a含量在12-24-48和72 h逐渐升高,两两比较差异均有统计学意义(P < 0.05)。结论:miR-27a在DDP诱导的SPC-A1细胞凋亡中表达升高,提示其可能参与细胞凋亡调控;对miR-27a进行定量检测有望用于对肺腺癌化疗疗效的评估。

    Abstract:

    Objective:To investigate the expression changes of miR-27a in human lung adenocarcinoma cell line SPC-A1 after cisplatin chemotherapy in virto,and to discuss its relationship with cell apoptosis and clinical significance. Methods:SPC-A1 cells were cultured in vitro and treated with DDP at 2.5 μg/ml for 12,24,48 and 72 h. Cell morphology was observed by phase contrast microscope,as well as cell apoptosis was detected by flow cytometry;expression changes of miR-27a both in cultural supernatants and SPC-A1 cells were analyzed by real-time PCR. Results:SPC-A1 cells showed morphological changes of apoptosis in the DDP (2.5 μg/ml) group. The apoptotic rates of SPC-A1 cells at 48 and 72 h in the DDP (2.5 μg/ml) group[(59.3 ± 2.5)% and (76.4 ± 3.1)%,respectively] were higher than that in the control group (P < 0.05). PCR analysis demonstrated that the levels of miR-27a were significantly increased in the DDP (2.5 μg/ml) group both in cultural supernatants and SPC-A1 cells compared to the level of the control group(P < 0.05). Conclusion:The level of miR-27a raised in human lung adenocarcinoma cell line SPC-A1 after cisplatin chemotherapy in virto. Quantitative analysis of miR-27a may have important value for chemotherapy monitoring of lung adenocarcinoma.

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朱善军,韩 月,徐 娟,谢而付,陈 丹,张美娟,张 燕,娄鉴芳,曹 艳,许雨乔,孙瑞红,王 芳,潘世扬.顺铂诱导SPC-A1人肺腺癌细胞凋亡过程中miR-27a的表达及意义[J].南京医科大学学报(自然科学版),2013,(7):873-876

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  • 收稿日期:2013-02-08
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  • 在线发布日期: 2013-07-09
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