文章摘要
曹 娜,张 浩,盛燕辉,杨 荣,孔祥清.Nodal信号通路相关基因NOMO1在斑马鱼早期胚胎发育过程中的表达[J].南京医科大学学报,2013,(9):1186~1190
Nodal信号通路相关基因NOMO1在斑马鱼早期胚胎发育过程中的表达
The expression pattern of Nodal signaling pathway gene NOMO1 during early embryonic development in zebrafish
投稿时间:2013-04-13  
DOI:10.7655/NYDXBNS20130903
中文关键词: 斑马鱼  NOMO1基因  基因克隆  Nodal 信号通路
英文关键词: zebrafish  NOMO1 gene  gene cloning  Nodal signaling
基金项目:国家自然科学基金青年科学基金资助(30901473)
作者单位
曹 娜 南京医科大学第一附属医院心血管内科,江苏 南京 210029 
张 浩 南京医科大学第一附属医院心血管内科,江苏 南京 210029 
盛燕辉 南京医科大学第一附属医院心血管内科,江苏 南京 210029 
杨 荣 南京医科大学第一附属医院心血管内科,江苏 南京 210029 
孔祥清 南京医科大学第一附属医院心血管内科,江苏 南京 210029 
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中文摘要:
      目的:研究斑马鱼NOMO1基因在早期胚胎发育过程中的时空表达情况。方法:首先克隆斑马鱼NOMO1同源基因全长cDNA,然后运用半定量RT-PCR技术初步检测NOMO1基因在斑马鱼早期胚胎的表达情况,最后通过原位杂交技术检测NOMO1基因在斑马鱼早期胚胎发育过程中的时空表达情况。结果:通过半定量RT-PCR及原位杂交技术显示NOMO1从受精卵时期开始即有表达,在24 hpf(受精后24 h)之前,NOMO1基因表达广泛,并主要集中在中?内胚层。从24 hpf开始其表达主要集中于神经系统如前脑?中脑?小脑?脊索前板?眼和耳囊等处。在心脏处也有少量表达。结论:NOMO1基因对斑马鱼早期胚胎发育具有一定的作用,推测NOMO1基因影响早期心脏发育。
英文摘要:
      Objective:To explore the expression pattern of zebrafish gene NOMO1 during early embryonic development. Methods:The zebrafish ortholog of the mammalian NOMO1 cDNA was cloned,and semi-quantitative RT-PCR was used to preliminarily detect the expression level of NOMO1 during the zebrafish embryonic development. At the last step,the expression pattern of zebrafish NOMO1 among the early stage of embryonic development was described by whole in situ hybridization. Results:cDNA of zebrafish NOMO1 is 3 924 bp,which contains a complete coding sequence of 3 626 bp. The expression of zebrefish NOMO1 is widely distributed from fertilization to 24 hpf(hours-post-fertilization),which is most abundantly transmitted in the endoderm and mesendoderm. After 24 hpf,the expression mainly concentrates on the nervous system,such as prosencephalon,mensencephalon,cerebellum,precordal plate,otic vesicles,eyes,and at somewhat lower level in heart. Conclusion:According to the expression pattern of NOMO1,NOMO1 may be required for the development of the zebrafish embryo, and influence the heart development during the early period.
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