文章摘要
曹 怡,陈 榕,邹大进.慢性能量限制对小鼠下丘脑食欲调节神经肽mRNA表达的影响[J].南京医科大学学报,2013,(9):1196~1201
慢性能量限制对小鼠下丘脑食欲调节神经肽mRNA表达的影响
Effects of chronic energy restricted diet on the mRNA expression of hypothalamic neuropeptides in mice
投稿时间:2013-04-17  
DOI:10.7655/NYDXBNS20130905
中文关键词: 肥胖  体重反弹  下丘脑  神经肽  能量限制
英文关键词: obesity  weight regain  hypothalamus  neuropeptide  energy restrict
基金项目:
作者单位
曹 怡 中国人民解放军北京军区总医院内分泌科,北京 100700 
陈 榕 第二军医大学附属长海医院内分泌科,上海 200433 
邹大进 第二军医大学附属长海医院内分泌科,上海 200433 
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中文摘要:
      目的:探讨小鼠下丘脑弓状核促进食欲和抑制食欲神经肽在慢性能量限制饮食后的变化。方法:将24只8周龄雄性C57BL/6J小鼠随机分为两组(每组12只),分别给予高脂和普通饲料喂养,8周后每组各取6只给予能量限制饮食(正常饮食量的85%),另6只继续给予高脂或普通饲料自由饮食,6周后处死。喂养过程中监测小鼠体重,处死后取下丘脑组织检测4种神经肽mRNA表达水平。结果:6周的能量限制逆转了小鼠的体重增加?脂肪蓄积。且肥胖逆转后,下丘脑弓状核促进食欲的神经肽Y(NPY)和刺鼠色蛋白相关蛋白(AgRP)mRNA的表达较肥胖小鼠显著增高(P < 0.001),而抑制食欲的前阿片黑素细胞皮质激素(POMC)和可卡因苯丙胺调节转录物(CART)mRNA没有明显变化。结论:慢性能量限制饮食能够引起体重减轻,但同时上调下丘脑促进食欲的神经肽,而后者可能是节食减重后体重反弹的一个重要促成因素。
英文摘要:
      Objective:To investigate the role of orexigenic and anorectic neuropeptides in hypothalamic arcuate nucleus in chronic energy restricted mice. Methods:Twenty-four C57BL/6J male mice were randomly divided into high fat diet group and normal diet group. After 8 weeks,6 mice of each group were given energy restricted diet (85% of normal or high fat diet). The mice were sacrificed after another 6 weeks. The body weight was determined every week during the 14 weeks feeding study. The neuropeptides mRNA expression levels from hypothalamic tissue were analyzed after sacrifice. Results:The energy restricted diet reversed obesity,and increased NPY and AgRP mRNA expression of hypothalamic arcuate nucleus after 6 weeks(P < 0.001). POMC and CART mRNA expression were not changed significantly. Conclusion:Chronic energy restriction leads to weight loss,but it also up-regulates hypothalamic orexigenic peptides,which may be an important contributing factor to weight regain after the energy restricted diet.
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