文章摘要
周 红,刘弈彤,梁小燕,戚晓红.硫化氢抑制局部肾素水平影响动脉粥样硬化的研究[J].南京医科大学学报,2013,(9):1208~1213
硫化氢抑制局部肾素水平影响动脉粥样硬化的研究
Hydrogen sulfide prevents atherosclerosis development via inhibition of local renin in the aortic tissue in the high fat treated rats
投稿时间:2013-04-19  
DOI:10.7655/NYDXBNS20130907
中文关键词: 动脉粥样硬化  硫化氢  肾素  肾素-血管紧张素系统
英文关键词: atherosclerosis  hydrogen sulfide  renin  renin angiotensin system
基金项目:江苏高校优势学科建设工程资助项目(JX10131801070,JX10131801095)
作者单位
周 红 南京医科大学基础医学实验教学示范中心,江苏 南京 210029 
刘弈彤 新加坡国立大学药理学系,新加坡 117597 
梁小燕 南京医科大学病理生理学系,江苏 南京 210029 
戚晓红 南京医科大学病理生理学系,江苏 南京 210029 
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中文摘要:
      目的:观察硫化氢(H2S)是否通过抑制局部肾素活性影响动脉粥样硬化(AS)。方法:用高脂饲料结合维生素D3的方法建立大鼠动脉粥样硬化模型,治疗组给予腹腔注射硫氢化钠(NaHS)56 ?滋mol/(kg?d),共计12周。用HE?油红O染色观察血管病理变化和脂质浸润,放免法检测血浆及组织肾素活性,免疫组化法观察血管局部肾素的表达。结果:模型组血浆总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)水平明显升高,血浆和动脉组织H2S水平显著降低,动脉组织肾素水平明显增高。NaHS组血脂和血浆H2S水平有所改善,动脉组织肾素水平降低,AS斑块和脂质浸润减少。结论:局部肾素在AS中发挥重要作用,H2S能通过抑制动脉局部肾素表达产生抗AS作用。
英文摘要:
      Objective:To investigate whether hydrogen sulfide(H2S) can prevent atherosclerotic development via suppression of local renin activity. Methods:Rats in atherosclerotic model group (MG) were fed with high fat diet. H2S treatment were performed with NaHS 56 ?滋mol/(kg?d) i.p. for 12 weeks. Aorta morphological changes were observed by HE and oil red O staining. The renin acitivity in plasma and tissue was detected by radio-immunity and the renin express in tissue was observed by immunostaining. Results:Endogenous H2S level in both plasma and aortic tissue was significantly decreased in MG,but the renin level was obviously increased in the aortic tissue. Exogenous application of NaHS prevented the elevated levels of serum lipids including total cholesterol,triglyceride,high-density lipoprotein-cholesterol,and low-density lipoprotein-cholesterol,and decreased the renin level in aortic tissue. Histology staining showed that NaHS treatment attenuated plaque formation and decreased lipid infiltration and accumulation in the plaque area in high fat treated rats. Conclusion:These data suggest that local renin is important in the AS development. Exogenous NaHS supplementation significantly prevents escalation of tissue renin expression. Hence,the present study presents a novel mechanism for the protective effect of H2S on atherosclerotic progression via inhibition of local renin expression in the aortic tissues.
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