胃癌组织和血浆中氨基酸及脂类的代谢变化研究
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The research on metabolic changes of amino acid and lipids in tissues and plasma of gastric cancer
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    摘要:

    目的:研究氨基酸和脂类在胃癌组织和血浆中的代谢变化,揭示胃癌组织和血浆之间的代谢联系。方法:应用基于气相色谱-飞行时间质谱(GC/TOFMS)的测定技术,检测20份慢性胃炎组织-17份胃癌组织-15份慢性胃炎患者血浆-15份胃癌患者血浆和15份胃癌术后患者血浆中的氨基酸和脂类的水平,结合t检验分析数据。结果:胃癌组织内氨基酸水平较慢性胃炎组织升高或相当,尤以半胱氨酸(P = 0.007)-2-氨基己二酸 (P = 0.045)-谷氨酰胺(P = 0.047)明显;胃癌患者血浆中的氨基酸水平低于慢性胃炎患者血浆,尤其半胱氨酸(P = 0.029)-丝氨酸(P = 0.013)-甘氨酸(P = 0.043)。与慢性胃炎组织相比,胃癌组织中3-羟基丁酸(P = 0.003)-十六碳烯酸(P = 0.030)-庚酸(P = 0.047)明显增高,胆固醇(P = 0.003)明显降低。胃癌术后患者血浆中氨基酸和脂类的水平向慢性胃炎状态恢复。结论:本研究揭示了胃癌组织中氨基酸和脂类代谢异常影响了血浆中的氨基酸和脂类;基于GC/TOFMS的测定方法是研究胃癌代谢的合适平台。

    Abstract:

    Objective:To investigate metabolic changes of amino acid and lipids in tissues and plasma of gastric cancer(GC),and to reveal the metabolic link between tissues and plasma of gastric cancer. Methods:The levels of amino acid and lipids were measured in 20 chronic superficial gastritis (CSG) tissue samples,17 GC tissue samples,15 CSG plasma samples,15 GC plasma samples and 15 postoperative GC plasma samples by means of gas chromatography-time of flight mass spectrometry (GC/TOFMS)-based technique,and t-test was employed to analyze data. Results:The levels of amino acids in GC tissue were generally elevated or equal to CSG tissue,especially cysteine(P = 0.007),and 2-aminoadipic acid (P = 0.045)and glutamine(P = 0.047),and the levels of amino acid in GC plasma were generally lower than that in CSG plasma,especially cysteine(P = 0.029),serine(P = 0.013)and glycine(P = 0.043). Compared to CSG tissue,the levels of 3-hydroxybutyric acid (P = 0.003),hexadecenoic acid(P = 0.030) and heptanoic acid(P = 0.047) were up-regulated,while cholesterol (P = 0.003)was down-regulated in GC tissue. The levels of amino acid and lipids metabolites in postoperative GC plasma were restored towards CSG plasma. Conclusion:The study demonstrates that the abnormal metabolism of amino acid and lipids in GC tissue affects the metabolism of amino acid and lipids in plasma,and suggestes GC/TOF-based techniques is a suitable tool for gastric cancer metabolism investigations.

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孙 敏,于莲珍,阿基业,王海洋,徐 瑾,李柯蓓,邱洪清.胃癌组织和血浆中氨基酸及脂类的代谢变化研究[J].南京医科大学学报(自然科学版),2013,(9):1251-1255

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  • 收稿日期:2013-02-03
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  • 在线发布日期: 2013-09-18
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