文章摘要
张 婷,何 松,陈旭东,陆晓云,杨书云,周建云,张建兵,潘红佳,严 桥.甲状腺乳头状癌伴神经内分泌分化的免疫组化、超微结构及免疫金标记研究[J].南京医科大学学报,2013,(11):1529~1536
甲状腺乳头状癌伴神经内分泌分化的免疫组化、超微结构及免疫金标记研究
Immunohistochemical,ultrastructural analysis and immunogold labelling of papillary thyroid carcinoma with neuroendocrine differentiation
投稿时间:2013-04-30  
DOI:10.7655/NYDXBNS20131109
中文关键词: 甲状腺乳头状癌  神经内分泌分化  免疫组织化学  透射电镜  免疫金标
英文关键词: papillary thyroid carcinoma  neuroendocrine differentiation  immunohistochemistry  transmission electron microscope  immunogold labelling
基金项目:南通市社会发展科技计划(S5010)
作者单位
张 婷 湖州师范学院医学院病理教研室,浙江 湖州 313000 
何 松 南通大学附属肿瘤医院病理科,江苏 南通 226361 
陈旭东 南通大学附属肿瘤医院病理科,江苏 南通 226361 
陆晓云 南通大学附属肿瘤医院病理科,江苏 南通 226361 
杨书云 南通大学附属肿瘤医院病理科,江苏 南通 226361 
周建云 南通大学附属肿瘤医院病理科,江苏 南通 226361 
张建兵 浙江大学医学院附属邵逸夫医院病理科,浙江 杭州 310016 
潘红佳 嘉兴市第二医院病理科,浙江 嘉兴 314000 
严 桥 南通大学医学院病理教研室,江苏 南通 226001 
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中文摘要:
      目的:探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)的神经内分泌(NE)分化与临床病理形态学特征及预后因素的关系。方法:手术切除PTC 103例,行HE染色后光镜下观察?免疫组织化学染色后光镜及透射电镜检查?免疫金标记后电镜观察PTC临床病理形态学?光镜及电镜下NE标记(NSE?CgA?Syn)表达及电镜CgA 标记的致密核心颗粒表达;分析上述NE标记表达与临床病理参数及预后因素的关系。结果:PTC表达NSE 64例(62.14%),与肿瘤直径呈正相关(P < 0.05);表达CgA25例(24.27%),与肿瘤分化程度呈负相关,与核内包涵体呈正相关(P < 0.05);Syn 均呈阴性;NSE及CgA共同表达23例(22.33%)。透射电镜PTC致密颗粒检出率为18.2%(12/66);PTC细胞分化程度?肿瘤直径与NE分化有关(P < 0.05);免疫金标记电镜观察CgA免疫金颗粒3例(2.91%)。CgA定位于PTC癌细胞胞浆内的NE颗粒边缘呈散在分布。结论:PTC中NE分化细胞可能来源于滤泡上皮细胞;NE分化与PTC瘤体直径?细胞核形态改变及癌细胞分化程度有关,与预后无关。
英文摘要:
      Objective:To investigate the correlation between neuroendocrine(NE)differentiation and clinico-pathological features,prognostic factors of papillary thyroid carcinoma(PTC). Methods:The tissues of 103 PTC cases were amaylzed by HE staining,immunohisto-chemistry,immunogold labelling,biomarkers expression of neuroendocrine differentiation. Results:Thyroglobulin was positively expressed in PTC,while Calcitonin was negative. The larger tumor size of the cases with PTC accompanied with a higher NSE expression(P < 0.05). The higher degree of differentiation accompanied with a lower rate of CgA expression. The cases with nuclear inclusions showed obviously increased expression of CgA(P < 0.05). The degree of PTC cell differentiation and tumor size were associated with NE differentiation under TEM(P < 0.05). The CgA immunogold particles were scattered at the internal edge of NE particles within the cytoplasm of PTC cells. Conclusion:NE differentiation could occur in PTC,and NE differentiated cells could be derived from follicular epithelial cells instead of C cells. NE differentiation is correlated with tumor size,morphological change of nuclei and degree of differentiation in PTC. NE differentiation has no significant correlation with the prognosis of PTC.
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