Objective:To investigate the expression of FOXC1 in non-small cell lung cancer(NSCLC)and its prognostic value. Methods:The tissue microarrays containing 202 NSCLC tissues and 63 normal lung tissues were constructed. The expression of FOXC1 was detected in these tissues by immunohistochemical method. The relationship between expression of FOXC1 and NSCLC’s clinicopathological and survival parameters were evaluated. Results:The expression of FOXC1(60.87%) in the NSCLC tissues was significantly higher than that in the normal group(25.40%)(P < 0.000 1). In the group of NSCLC,FOXC1 expression was significantly correlated with TNM stage and lymph node metastasis. Kaplan-Meier survival analysis indicated that the survival rate was markedly lower in the patients with high level of FOXC1 than those with low level of FOXC1(P < 0.001).Conclusion:The expression of FOXC1 in NSCLC was significantly correlated with the development and progression of the neoplasms. FOXC1 may play a important role in the evaluation of the prognosis of NSCLC patients.