文章摘要
朱 楠,张积森,荆珏华,钱 军,仇汪宝.MicroRNA-199a-3p对人骨肉瘤细胞凋亡的影响[J].南京医科大学学报,2014,(2):159~163
MicroRNA-199a-3p对人骨肉瘤细胞凋亡的影响
Effects of microRNA-199a-3p on apoptosis of human osteosarcoma cells
投稿时间:2013-07-13  
DOI:10.7655/NYDXBNS20140207
中文关键词: 骨肉瘤  凋亡  microRNA-199a-3p  转染
英文关键词: osteosarcoma  apoptosis  microRNA-199a-3p  transfection
基金项目:安徽医科大学校科研基金(2013xkj033)
作者单位
朱 楠 安徽医科大学第二附属医院骨科,安徽 合肥 230601 
张积森 安徽医科大学第二附属医院骨科,安徽 合肥 230601 
荆珏华 安徽医科大学第二附属医院骨科,安徽 合肥 230601 
钱 军 安徽医科大学第二附属医院骨科,安徽 合肥 230601 
仇汪宝 安徽医科大学第二附属医院骨科,安徽 合肥 230601 
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中文摘要:
      目的:探讨microRNA-199a-3p (miR-199a-3p)对人骨肉瘤细胞凋亡的影响?方法:用合成的成熟miR-199a-3p序列模拟物(miR-199a-3p mimics)转染人骨肉瘤细胞(U2-OS),以阴性对照物序列(NC mimics)转染细胞作为阴性对照?转染后应用实时定量逆转录聚合酶链反应(qRT-PCR)检测各组细胞miR-199a-3p的表达量,Western blot法检测各组细胞中髓样细胞白血病-1(MCL-1)蛋白的表达水平及多聚腺苷二磷酸核糖聚合酶(PARP)的剪切情况,利用流式细胞仪检测各组细胞的凋亡率,并对实验结果进行统计学分析?结果:与对照组相比,转染miR-199a-3p mimics的实验组细胞中,miR-199a-3p的表达量明显升高,MCL-1蛋白的表达降低,PARP蛋白的剪切水平增加,细胞的凋亡率增高,差异均有统计学意义(P < 0.05)?结论:miR-199a-3p可以有效促进骨肉瘤细胞的凋亡?
英文摘要:
      Objective:MicroRNA-199a-3p (miR-199a-3p) is down-regulated in many kinds of tumors,including osteosarcoma. This study was aimed to investigate the effect of miR-199a-3p on apoptosis of human osteosarcoma cells (U2-OS). Methods:Human osteosarcoma cells(U2-OS) were transfected with miR-199a-3p mimics or negative control mimics (NC mimics) as negative control. MiR-199a-3p expression level was detected by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). Expression level of myeloid cell leukemia-1 (MCL-1) protein and cleavage of poly ADP-ribose polymerase(PARP) were detected by Western blot. Cell apoptosis was detected by flow cytometry. Results:Compared with control group,miR-199a-3p expression level of experiment group was significantly up-regulated,MCL-1 protein expression was reduced,cleavage level of PARP was increased and cell apoptosis rate of experiment group was significantly increased. The differences between experimental and control groups have statistical significance(P < 0.05). Conclusion:MiR-199a-3p can dramatically promote apoptosis of human osteosarcoma cells.
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