文章摘要
曹 艳,王 鹏,娄鉴芳,李大千,吴 蕾,陈 丹,谢而付,顾 兵,徐华国,王 芳,徐 建,潘世扬.miR-638对人肺腺癌细胞凋亡的影响[J].南京医科大学学报,2014,(3):287~290
miR-638对人肺腺癌细胞凋亡的影响
Impact of miR-638 on apoptosis of lung adenocarcinoma cells
投稿时间:2013-12-19  
DOI:10.7655/NYDXBNS20140303
中文关键词: 凋亡  miR-638  肺腺癌
英文关键词: apoptosis  miR-638  lung adenocarcinoma
基金项目:国家自然科学基金(81371894,81302531, 81272324,81201359,81101322);江苏省实验诊断学重点实验室(XK201114);江苏高校优势学科建设工程基金项目;教育部博导基金(20113234110012);国家临床重点专科建设项目
作者单位
曹 艳 南京医科大学第一附属医院检验学部,江苏 南京 210029 
王 鹏 南京医科大学第一附属医院检验学部,江苏 南京 210029 
娄鉴芳 南京医科大学第一附属医院检验学部,江苏 南京 210029 
李大千 南京医科大学第一附属医院检验学部,江苏 南京 210029 
吴 蕾 南京医科大学第一附属医院检验学部,江苏 南京 210029 
陈 丹 南京医科大学第一附属医院检验学部,江苏 南京 210029 
谢而付 南京医科大学第一附属医院检验学部,江苏 南京 210029 
顾 兵 南京医科大学第一附属医院检验学部,江苏 南京 210029 
徐华国 南京医科大学第一附属医院检验学部,江苏 南京 210029 
王 芳 南京医科大学第一附属医院检验学部,江苏 南京 210029 
徐 建 南京医科大学第一附属医院检验学部,江苏 南京 210029 
潘世扬 南京医科大学第一附属医院检验学部,江苏 南京 210029 
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中文摘要:
      目的:探讨miR-638在肺腺癌中的表达及其与肺腺癌细胞凋亡的关系?方法:应用real-time RT-PCR的方法检测了miR-638在肺腺癌细胞株SPC-A1中的表达情况,并采用脂质体法将miR-638 模拟物瞬时转染入SPC-A1?实验设置空白对照组?无关miRNA阴性对照组和miR-638转染组,转染后在荧光显微镜下观察转染效率,实时荧光定量RT-PCR检测miR-638的表达,流式细胞术检测各组细胞凋亡率?结果:与正常细胞相比,miR-638在肺腺癌细胞中低表达?SPC-A1细胞转染miR-638模拟物后,细胞凋亡率相对于空白组和阴性对照组显著升高(P < 0.05)?结论:miR-638在肺腺癌中低表达,且能够促进肺腺癌细胞凋亡,可作为后续肺癌生物治疗的新分子靶标?
英文摘要:
      Objective:To investigate expression of miR-638 and effects of miR-638 on apoptosis of lung adenocarcinoma cell line(SPC-A1). Methods:The expression of miR-638 in lung adenocarcinoma cell line was detected by real-time RT-PCR. Mimics of mir-638 were transiently transfected into SPC-A1 by using lipofectamine method. SPC-A1 cells were transfected with miR-638 mimics or non-special oligonucleotides(as negative control)or nothing(as blank control). After transfection,transfection efficiency was observed by fluorescence microscope. MiR-638 levels were detected by real-time quantitative RT-PCR. Cell apoptosis was detected by flow cytometry. Results:We observed that miR-638 was significantly inhibited in lung adenocarcinoma cells compared with that in normal cells. Cell apoptosis rate was increased significantly in cells transfected with miR-638(P <0.05)compared with negative and blank control cells. Conclusion:MiR-638 was poorly expressed in lung adenocarcinoma cells,and it could dramatically promote apoptosis of lung adenocarcinoma cells,thus provides a new target for the use of miR-638 in lung cancer biotherapy.
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