文章摘要
陈嘉彤,王稚英,王雪松.细菌内毒素对钛板上人牙龈成纤维细胞生物学形态及OPG表达的影响[J].南京医科大学学报,2014,(3):310~313
细菌内毒素对钛板上人牙龈成纤维细胞生物学形态及OPG表达的影响
Effects of bacterial endotoxin on bio-morphology and OPG level of human gingival fibroblasts on titanium plate
投稿时间:2013-07-10  
DOI:10.7655/NYDXBNS20140307
中文关键词: 细菌内毒素  人牙龈成纤维细胞  骨保护蛋白
英文关键词: bacterial endotoxin  human gingival fibroblasts  osteoprotegerin
基金项目:
作者单位
陈嘉彤 辽宁医学院附属第二医院口腔种植中心,辽宁 锦州 121000 
王稚英 辽宁医学院附属第二医院口腔种植中心,辽宁 锦州 121000 
王雪松 辽宁医学院附属第二医院口腔种植中心,辽宁 锦州 121000 
摘要点击次数: 800
全文下载次数: 461
中文摘要:
      目的:通过研究不同浓度的细菌内毒素对钛板上人牙龈成纤维细胞及其产物骨保护蛋白(osteoprotegerin,OPG)的影响,了解内毒素对种植体软组织以及骨组织愈合的作用?方法: 人牙龈组织块培养并用免疫荧光染色方法进行组织来源鉴定,用0?10?100 μg/ml浓度的细菌内毒素分别刺激钛板上的牙龈成纤维细胞,扫描电镜观察牙龈成纤维细胞的形态,并用ELISA法检测OPG的含量并进行统计学分析?结果:10 μg/ml细菌内毒素刺激的牙龈成纤维细胞较正常组细胞伪足增多,细胞基质分泌增加,而100 μg/ml组的细胞数量及基质分泌明显减少?ELISA法表明低浓度条件下,OPG的含量随着细菌内毒素浓度增多而增加,但100 μg/ml 组OPG含量显著降低?结论:低浓度细菌内毒素可以促进牙龈成纤维细胞生长及分泌细胞基质OPG,而高浓度细菌内毒素则抑制牙龈成纤维细胞增殖且OPG表达降低?
英文摘要:
      Objective:To study the effects of bacterial endotoxin with different concentrations on human gingival fibroblast (HGF) on titanium plate and its product osteoprotegerin(OPG), and to understand the healing function of endotoxin on soft tissue and bone tissue of dental implants. Methods:Tissue block culture method was applied to culture human gingiva and gingival fibroblasts were identified by immunofluorescence staining method. Endotoxin with different concentrations of 0,10 and 100 μg/ml were used to stimulate HGF on the titanium plate and HGF morphology was observed by scanning electron microscope(SEM). ELISA was applied to detect the OPG level. Results:Compare with the normal group,the HGF stimulated by endotoxin of 10 μg/ml had more cellular pseudopods and cellular matrix secretion was increased. However, in the 100 μg/ml endotoxin stimulated group,number of cells and cellular matrix secretion were decreased significantly. ELISA method demonstrated that under the condition of low concentration, OPG increased with the increase of endotoxin concentration. But the OPG level of 100 μg/ml group decreased significantly. Conclusion:Low concentration of bacterial endotoxin can promote growth of HGF and secretion of cellular matrix OPG;whereas high concentration of bacterial endotoxin can inhibit growth of gingival fibroblasts and decrease OPG level.
查看全文   查看/发表评论  下载PDF阅读器
关闭