盐酸埃克替尼联合1α,25-二羟基维生素D3对人肺腺癌A549细胞增殖及EGFR表达的影响
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Combination of icotinib hydrochloride and 1α,25-dihydroxyxitamin D3 on proliferation and expression of EGFR of human lung adenocarcinoma cell line A549
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    目的:研究盐酸埃克替尼和1α,25-二羟基维生素D3单药及联合用药对人肺腺癌A549细胞增殖及表皮生长因子受体(EGFR)表达的影响-方法:盐酸埃克替尼和1α,25-二羟基维生素D3单药及联合用药处理A549细胞后,用CCK-8法检测细胞增殖抑制率的变化;用Western blot法及RT-PCR法分别检测细胞EGFR蛋白及EGFR mRNA表达的变化-结果:与单药盐酸埃克替尼组和1α,25-二羟基维生素D3组相比,联合用药组具有更强的细胞增殖抑制作用(P均< 0.05);联合用药组及1α,25-二羟基维生素D3单药组EGFR蛋白及EGFR mRNA表达水平较阴性对照组及盐酸埃克替尼单药组有明显升高(P均< 0.05)-结论:盐酸埃克替尼与1α,25-二羟基维生素D3联合用药对人肺腺癌A549细胞具有协同抗增殖作用;其机制可能与1α,25-二羟基维生素D3升高了EGFR mRNA及EGFR蛋白的表达,从而更有利于盐酸埃克替尼发挥抗肿瘤作用有关-

    Abstract:

    Objective:To study the effects of icotinib hydrochloride and 1α,25-dihydroxyxitamin D3 on proliferation and expression of EGFR of human lung adenocarcinoma cell line A549. Methods:The human lung adenocarcinoma A549 cell was cultured and treated by icotinib hydrochloride,1α,25-dihydroxyxitamin D3 and combination of these two medicines at different concentrations and time intervals. The CCK-8 assay was applied to analyze the proliferation inhibition rate. The expressions of EGFR total protein and EGFR mRNA were detected by western blotting and RT-PCR,respectively. Results:Compared with the single drug group,there was synergistic effect observed in the groups treated with icotinib hydrochloride and 1α,25-dihydroxyxitamin D3 along(P < 0.05). Compared with the control group and the group treated with icotinib hydrochloride alone,the expressions of EGFR total protein and EGFR mRNA of the group treated with 1α,25-dihydroxyxitamin D3 alone or the combination group were elevated dramatically(P < 0.05). Conclusion:There was synergistic effect in the cells treated with combination icotinib hydrochloride with 1α,25-dihydroxyxitamin D3. This may attribute to up-regulat of the expressions of EGFR mRNA and EGFR total protein by 1α,25-dihydroxyxitamin D3,and further to the anti-tumor effect of icotinib hydrochloride.

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冯应勤,朱金峰,何兴端,沈浩军,梅家转,文建国,鲁 培.盐酸埃克替尼联合1α,25-二羟基维生素D3对人肺腺癌A549细胞增殖及EGFR表达的影响[J].南京医科大学学报(自然科学版),2014,(5):563-567

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  • 收稿日期:2013-12-17
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  • 在线发布日期: 2014-05-21
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