文章摘要
冯应勤,朱金峰,何兴端,沈浩军,梅家转,文建国,鲁 培.盐酸埃克替尼联合1α,25-二羟基维生素D3对人肺腺癌A549细胞增殖及EGFR表达的影响[J].南京医科大学学报,2014,(5):563~567
盐酸埃克替尼联合1α,25-二羟基维生素D3对人肺腺癌A549细胞增殖及EGFR表达的影响
Combination of icotinib hydrochloride and 1α,25-dihydroxyxitamin D3 on proliferation and expression of EGFR of human lung adenocarcinoma cell line A549
投稿时间:2013-12-17  
DOI:10.7655/NYDXBNS20140506
中文关键词: 盐酸埃克替尼  1α,25-二羟基维生素D3  表皮生长因子受体  细胞增殖  基因表达
英文关键词: icotinib hydrochloride  1α,25-dihydroxyxitamin D3  epidermal growth factor receptor  cell proliferation  gene expression
基金项目:
作者单位
冯应勤 郑州大学第一附属医院肿瘤科,河南 郑州 450000 
朱金峰 郑州大学第一附属医院肿瘤科,河南 郑州 450000 
何兴端 郑州大学第一附属医院肿瘤科,河南 郑州 450000 
沈浩军 郑州大学第一附属医院肿瘤科,河南 郑州 450000 
梅家转 郑州人民医院肿瘤科,河南 郑州 450000 
文建国 河南省高等学校临床医学重点学科开放实验室,河南 郑州 450000 
鲁 培 郑州大学第一附属医院肿瘤科,河南 郑州 450000 
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中文摘要:
      目的:研究盐酸埃克替尼和1α,25-二羟基维生素D3单药及联合用药对人肺腺癌A549细胞增殖及表皮生长因子受体(EGFR)表达的影响?方法:盐酸埃克替尼和1α,25-二羟基维生素D3单药及联合用药处理A549细胞后,用CCK-8法检测细胞增殖抑制率的变化;用Western blot法及RT-PCR法分别检测细胞EGFR蛋白及EGFR mRNA表达的变化?结果:与单药盐酸埃克替尼组和1α,25-二羟基维生素D3组相比,联合用药组具有更强的细胞增殖抑制作用(P均< 0.05);联合用药组及1α,25-二羟基维生素D3单药组EGFR蛋白及EGFR mRNA表达水平较阴性对照组及盐酸埃克替尼单药组有明显升高(P均< 0.05)?结论:盐酸埃克替尼与1α,25-二羟基维生素D3联合用药对人肺腺癌A549细胞具有协同抗增殖作用;其机制可能与1α,25-二羟基维生素D3升高了EGFR mRNA及EGFR蛋白的表达,从而更有利于盐酸埃克替尼发挥抗肿瘤作用有关?
英文摘要:
      Objective:To study the effects of icotinib hydrochloride and 1α,25-dihydroxyxitamin D3 on proliferation and expression of EGFR of human lung adenocarcinoma cell line A549. Methods:The human lung adenocarcinoma A549 cell was cultured and treated by icotinib hydrochloride,1α,25-dihydroxyxitamin D3 and combination of these two medicines at different concentrations and time intervals. The CCK-8 assay was applied to analyze the proliferation inhibition rate. The expressions of EGFR total protein and EGFR mRNA were detected by western blotting and RT-PCR,respectively. Results:Compared with the single drug group,there was synergistic effect observed in the groups treated with icotinib hydrochloride and 1α,25-dihydroxyxitamin D3 along(P < 0.05). Compared with the control group and the group treated with icotinib hydrochloride alone,the expressions of EGFR total protein and EGFR mRNA of the group treated with 1α,25-dihydroxyxitamin D3 alone or the combination group were elevated dramatically(P < 0.05). Conclusion:There was synergistic effect in the cells treated with combination icotinib hydrochloride with 1α,25-dihydroxyxitamin D3. This may attribute to up-regulat of the expressions of EGFR mRNA and EGFR total protein by 1α,25-dihydroxyxitamin D3,and further to the anti-tumor effect of icotinib hydrochloride.
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