文章摘要
黄玉晗,刘 佳,刘 思,武 侠,徐亚光,赵秀芬,钱 军,邢昌赢.氟伐他汀在大鼠腹膜透析模型中对腹膜的保护作用[J].南京医科大学学报,2014,(5):603~606
氟伐他汀在大鼠腹膜透析模型中对腹膜的保护作用
Protective effect of fluvastatin in peritoneal dialysis rats model
投稿时间:2014-01-09  
DOI:10.7655/NYDXBNS20140513
中文关键词: 腹膜透析  氟伐他汀  高糖腹透液  转化生长因子β1  纤维连接蛋白
英文关键词: peritoneal dialysis  fluvastatin  high-glucose peritoneal dialysate  transformating growth factor-β1  fibronectin
基金项目:国家自然科学基金(81170660);江苏省科技厅基础研究计划(自然科学基金)( BK2011849);江苏省卫生厅科研基金资助(H200317)
作者单位
黄玉晗 南京医科大学第一附属医院肾内科,江苏 南京 210029 
刘 佳 南京医科大学第一附属医院肾内科,江苏 南京 210029 
刘 思 南京医科大学第一附属医院肾内科,江苏 南京 210029 
武 侠 南京医科大学第一附属医院肾内科,江苏 南京 210029 
徐亚光 南京医科大学第一附属医院肾内科,江苏 南京 210029 
赵秀芬 南京医科大学第一附属医院肾内科,江苏 南京 210029 
钱 军 南京医科大学第一附属医院肾内科,江苏 南京 210029 
邢昌赢 南京医科大学第一附属医院肾内科,江苏 南京 210029 
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中文摘要:
      目的:探讨氟伐他汀(fluvastatin,Flu)对腹膜透析(peritoneal dialysis,PD)大鼠腹膜组织形态影响的初步研究?方法:通过SD雄性大鼠(体重180~200 g)腹腔注射4.25%浓度的葡萄糖腹透液的方式模拟腹膜透析?随机分为5个组:①正常对照组;②4.25%腹透液组;③单纯Flu组;④生理盐水组;⑤Flu干预的4.25%腹透液组?4周后留取各组大鼠壁层腹膜组织,光镜观察各组大鼠壁层腹膜形态学的变化,同时采用RT-PCR法测定各组大鼠腹膜转化生长因子-β1(transforming growth factor,TGF-β1)及纤维连接蛋白(fibronectin,FN)mRNA的表达?结果:光镜下HE染色显示4.25%腹透液组大鼠腹膜较正常对照组显著增厚,Flu干预组的壁层腹膜厚度薄于未干预组,生理盐水组和单纯Flu组大鼠腹膜的形态较正常对照组未见明显改变?4.25%腹透液组大鼠腹膜TGF-β1及FN mRNA的表达量均高于正常对照组(均P < 0.05),Flu干预组两者mRNA的表达量均明显低于未干预组(均P < 0.05),生理盐水组和单纯Flu组大鼠腹膜两者的mRNA表达量较正常组无明显统计学意义?结论:长期使用高糖腹透液会改变腹膜的结构,氟伐他汀在一定程度上对腹膜有保护作用,但具体机制仍需进一步研究?
英文摘要:
      Objective:To preliminarily investigate whether fluvastatin has a therapeutic role on the peritoneum in a rat model of peritoneal dialysis. Methods:A rat model of peritoneal dialysis was induced by a daily intraperitoneal infusion of 4.25% peritoneal dialysate. Thirty SD rats were randomly divided into five groups:normal control group(n = 6),4.25% peritoneal dialysate group(n = 6),saline group(n = 6),Flu group(n = 6),4.25% peritoneal dialysate combined with Flu group(n = 6). Rats were sacrificed 4 weeks after the first treatment and the peritoneal tissues were gathered for further analysis. A portion of peritoneal tissues were used for histological examination. The expressions of TGF-β1 and FN of each group were examined by RT-PCR. Results:HE stain showed that compared with normal control group,the peritoneal tissue thickness of 4.25% peritoneal dialysate group and 4.25% peritoneal dialysate combined with Flu group increased significantly. Compared with 4.25% peritoneal dialysate group,the peritoneal tissue thickness of 4.25% peritoneal dialysate combined with Flu group decreased obviously. There was no obvious statistical significance among saline group,Flu group and normal control group. RT-PCR showed that compared with normal control group,the expressions of TGF-β1 and FN in 4.25% peritoneal dialysate group increased apparently. The expressions of TGF-β1 and FN in 4.25% peritoneal dialysate combined with Flu group were significantly lower than 4.25% peritoneal dialysate group(P < 0.05). There was no obvious statistical significance among saline group,Flu group and normal control group. Conclusion:Long-term treatment with high-glucose peritoneal dialysate could change the structure of peritoneum. Fluvastatin could be used for protecting peritoneum,however,the specific mechanism need tobefurtherstudied.
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