文章摘要
顾俊菲,叶山东,汪 姗,孙文佳,胡圆圆.二甲双胍对大鼠肾小球系膜细胞NF-κB,ICAM-1表达的影响[J].南京医科大学学报,2014,(5):607~610
二甲双胍对大鼠肾小球系膜细胞NF-κB,ICAM-1表达的影响
Effects of metformin on expressions of nuclear factor-κB and intercellular adhesion molecule-1 in rat glomerular mesangial cells
投稿时间:2013-10-07  
DOI:10.7655/NYDXBNS20140514
中文关键词: 二甲双胍  核因子-κB  细胞间黏附分子-1
英文关键词: metformin  NF-κB  ICAM-1
基金项目:安徽省自然科学基金(11040606M161);安徽高校省级自然科学研究项目(KJ2011A157)
作者单位
顾俊菲 安徽医科大学附属省立医院内分泌科,安徽 合肥 230001 
叶山东 安徽医科大学附属省立医院内分泌科,安徽 合肥 230001 
汪 姗 安徽医科大学附属省立医院内分泌科,安徽 合肥 230001 
孙文佳 安徽医科大学附属省立医院内分泌科,安徽 合肥 230001 
胡圆圆 安徽医科大学附属省立医院内分泌科,安徽 合肥 230001 
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中文摘要:
      目的:观察二甲双胍对大鼠肾小球系膜细胞(mesangial cells, MCs)核因子-κB(nuclear factor-κB,NF-κB)?细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)表达的影响,探讨其肾脏保护机制?方法:体外培养MCs,随机分为正常糖组(NG)?高糖组(HG)及高糖 + 不同浓度二甲双胍组(M1:0.5 mmol/L;M2:1.0 mmol/L;M3:2.0 mmol/L)?培养48 h后收集各组细胞,采用荧光实时定量聚合酶链式反应法(real time-PCR)检测细胞NF-κB mRNA及ICAM-1 mRNA含量,蛋白质印迹法(Western blot)检测细胞NF-κBp65和ICAM-1蛋白表达水平?结果:①MCs可表达NF-κB?ICAM-1;②与NG组比较,HG组MCs NF-κB?ICAM-1mRNA和蛋白表达增强(P < 0.05);③与HG组比较,M3组 MCs NF-κB?ICAM-1mRNA相对表达量明显降低,差异有统计学意义(P < 0.05);④与HG组比较,M1组?M2组和M3组MCs NF-κBp65?ICAM-1蛋白表达量明显降低,差异有统计学意义(P < 0.01)?结论:二甲双胍可抑制肾小球系膜细胞NF-κB?ICAM-1 mRNA和蛋白表达,并具有一定的浓度依赖性,该作用可能是其肾脏保护机制之一?
英文摘要:
      Objective:To observe the effects of metformin on expressions of nuclear factor-κB and intercellular adhesion molecule-1 in cultured rat glomerular mesangial cells(MCs) and explore its reno-protective mechanisms. Methods:MCs were cultured in the medium with normal glucose(group NG), high glucose(group HG),and different concentrations of metformin (group M1, 0.5 mmol/L;group M2, 1.0 mmol/L;group M3, 2.0 mmol/L). After 48h exposure, MCs were collected for mRNA and protein expression of NF-κB, ICAM-1. The expression of NF-κB and ICAM-1 mRNA was analyzed by real time polymerase chain reaction. The expression of NF-κBp65 and ICAM-1 protein was visualized by western blotting . Results:①MCs expressed NF-κB and ICAM-1. ②After stimulated by high glucose, the levels of intracelluar NF-κB, ICAM-1 mRNA and protein expression were significantly increased compared with group NG(P < 0.05). ③Compared with group HG, the expression of intracellular NF-κB, ICAM-1 mRNA was significantly decreased in group M3(P < 0.05). ④Compared with group HG, the expression of intracellular NF-κBp65 and ICAM-1 protein was significantly decreased in group M1, M2 and M3 in a dose-dependent manner(P < 0.01). Conlusion:Metformin can suppress the expression of NF-κB and ICAM-1 in glomerular mesangial cells in a concentration-dependent mode, which may partly contribute to its reno-protection.
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