Objective:To compare the biomechanical aspects among the three different fixation methods of Tossy type III acromioclavicular joint dislocation,and to guide the clinical treatment. Methods:Six adult cadaveric specimens were used in the study (12 shoulders). and made to the Tossy type Ⅲ acromioclavicular joint dislocation model. The 12 shoulder were randomly divided into 3 groups with 4 cases in each group,and the three groups received titanium cable fixation,anchor screw fixation and clavicular hook plate fixation respectively. After fixation,MTS biological material testing system was used to test the degree of excursion of three kinds of internal fixation. Results:All of the three internal fixation methods can be anatomical reduction. Compared with clavicular hook plate internal fixation group,the degree of activity in other two groups is larger(P < 0.05),and the degree of separation is smaller (P < 0.05). Conclusion:Congared with Tossy type Ⅲ acromioclavicular joint dislocation,the degree of separation of itanium cable fixation and anchor screw fixation is small,and the degree of activity is moderate,more closer to normal physiological state.