文章摘要
孔亮亮,倪庆锋,卢叶挺,丁文周,孔连宝.miR-30c减轻肝细胞肝癌的侵袭与转移[J].南京医科大学学报,2014,(7):937~943
miR-30c减轻肝细胞肝癌的侵袭与转移
miR-30c attenutes invasion and metastasis of hepatocellular carcinoma
投稿时间:2014-02-10  
DOI:10.7655/NYDXBNS20140716
中文关键词: miR-30c  肝细胞性肝癌  肿瘤侵袭
英文关键词: miR-30c  hepatocellular carcinoma  tumor invasion
基金项目:卫生部医药发展项目(W2012Fz058)
作者单位
孔亮亮 南京医科大学第一附属医院肝脏外科,江苏 南京 210029 
倪庆锋 南京医科大学第一附属医院肝脏外科,江苏 南京 210030 
卢叶挺 南京医科大学第一附属医院肝脏外科,江苏 南京 210031 
丁文周 南京医科大学第一附属医院肝脏外科,江苏 南京 210032 
孔连宝 南京医科大学第一附属医院肝脏外科,江苏 南京 210033 
摘要点击次数: 629
全文下载次数: 500
中文摘要:
      目的:观察miR-30c/snail在肝细胞肝癌(hepatocellular carcinoma,HCC)中的表达并探讨其在肝癌侵袭中的作用?方法:定量PCR检测肝癌及肝癌细胞系中miR-30c/snail的mRNA表达水平,通过免疫组织化学和Western blot分别检测肝癌及肝癌细胞系中snail的蛋白表达水平,并在肝癌细胞株中转染miR-30c模拟物或抑制物,分析其对肝癌细胞侵袭的作用?结果:与无脉管转移组相比,miR-30c在有脉管转移组的HCC组织中表达明显下调,同时在高转移肝癌细胞株MHCC-97H中也有相似的结果?转染miR-30c模拟物可减少snail的表达,并减少MHCC-97H细胞的侵袭性,然而,转染miR-30c抑制剂则增加snail的蛋白水平,增加HepG2细胞的侵袭力?结论:上调miR-30c的表达通过直接抑制snail,可减弱HCC的侵袭性?一种新的针对miR-30c的治疗策略可能有利于伴侵袭转移的HCC患者?
英文摘要:
      Objective:To detect the expression of miR-30c/snail and the role of miR-30c in hepatocelluar carcinoma (HCC) invasion. Methods:mRNA expression of miR-30c/snail in HCC samples and cell lines were determined by real-time PCR,while protein expression of snail in HCC tissues and cells were detected by immunohistochemistry and Western blotting assays,respectively. Moreover,invasion of HCC cells was analyzed after transfection with miR-30c mimic or inhibitor. Results:We found that the miR-30c levels were obviously decreased in HCC with vascular metastasis compared with non-metastatic tissues,and the same results were found in the highly metastatic cell line MHCC-97H. Transfection with miR-30c mimic decreased snail expression and reduced the invasion of MHCC-97H cells;however,transfection with miR-30c inhibitor increased the protein level of snail and promoted the invasion of HepG2 cells. Conclusion:Upregulation of miR-30c expression attenuates the invasion of HCC by directly inhibiting snail. A new therapeutic strategy based on miR-30c may prove to be beneficial for HCC patients with invasion or metastasis.
查看全文   查看/发表评论  下载PDF阅读器
关闭