文章摘要
徐芳媛,朱 叶,王 坤,姜润秋,于莲珍.白介素-22在胃癌恶性转化中的作用[J].南京医科大学学报,2014,(7):944~949
白介素-22在胃癌恶性转化中的作用
The effect of interleukin-22 in malignant transformation of gastric cancer
投稿时间:2014-04-02  
DOI:10.7655/NYDXBNS20140717
中文关键词: 胃癌  癌前病变  恶性转化  白介素-22  白介素-22受体  信号转导与转录激活因子3
英文关键词: gastric cancer  precancerous lesions  malignant transformation  IL-22  IL-22R  STAT3
基金项目:江苏省卫生厅六大人才高峰项目(2011年)
作者单位
徐芳媛 南京医科大学第一附属医院消化科,江苏 南京 210029 
朱 叶 南京医科大学第一附属医院消化科,江苏 南京 210030 
王 坤 南京医科大学第一附属医院消化科,江苏 南京 210031 
姜润秋 南京医科大学第一附属医院消化科,江苏 南京 210032 
于莲珍 南京医科大学第一附属医院消化科,江苏 南京 210033 
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中文摘要:
      目的:观察处于胃癌恶性转化不同阶段的患者组织中白介素-22(interleukin-22,IL-22)?IL-22受体1(interleukin-22 receptor1,IL-22R1)和信号转导与转录激活因子3(signaling and transcriptional activation factor 3,STAT3)的表达,探讨IL-22在胃癌恶性转化过程中的作用?方法:收集慢性胃炎组织?癌前病变胃组织及胃癌组织各15例,用免疫组化和实时定量PCR(real-time PCR)的方法,测定3组中IL-22?IL-22R1及p-STAT3(S727)的分布及表达?结果:①IL-22主要分布在异形腺体上皮细胞和腺体间浸润的散在的淋巴细胞胞浆中,IL-22R1主要分布在异形腺体上皮细胞的胞浆中,p-STAT3则主要定位于异形腺体上皮细胞的胞核中;②胃癌前病变组及胃癌组中的IL-22?IL-22R1及p-STAT3蛋白的表达量都明显高于慢性胃炎对照组;同时,胃癌组中IL-22?IL22-R1及p-STAT3蛋白的表达量明显高于胃癌前病变组,差异均具有统计学意义(P < 0.001);③胃癌前病变组及胃癌组中的IL-22和IL22-R1的mRNA表达量都明显高于慢性胃炎对照组;胃癌组中IL-22和IL22-R1的mRNA表达量明显高于胃癌前病变组,差异具有统计学意义(P < 0.05)?结论:胃癌前病变及胃癌组织中,IL-22不仅可由浸润的免疫细胞分泌,还可由异形腺体上皮细胞自分泌产生?胃癌的恶性转化过程中,IL-22可能起着促进作用,且其促进作用可能通过IL-22-IL-22R1-STAT3通路完成?
英文摘要:
      Objective:To observe the expression of interleukin 22(IL-22), interleukin 22 receptor 1(IL-22R1) and signaling and transcriptional activation factor 3(STAT3) in the different stages of malignant transformation of gastric cancer, and to illustrate the effect of IL-22 in the process. Methods:The tissues from patients with chronic gastritis (15 cases),gastric precancerous lesions(15 cases) and gastric carcinoma (15 cases) were collected and examined for IL-22,IL-22R1 and p-STAT3 expression by immunohistochemistry and real-time PCR. Results:Immunohistochemical analysis showed that IL-22 was mainly located in the cytoplasm of gastric epithelial cells and infiltrated immune cells. IL-22R1 was mainly expressed in the cytoplasm of gastric epithelial cells, while the location of p-STAT3 was mainly in the nucleus of gastric epithelial cells. The expression of IL-22, IL-22R1 and p-STAT3 in precancerous lesions and gastric carcinoma were significantly more than that in chronic gastritis. At the same time, IL-22,IL-22R1 and p-STAT3 were significantly increased in gastric carcinoma compared with precancerous lesions(P < 0.001). The expression of IL-22 and IL-22R1 mRNA were higher in precancerous lesions and gastric carcinoma than in chronic gastritis, and were higher in gastric carcinoma than in precancerous lesions(P < 0.05). Conclusion:In the cells of precancerous lesions and gastric carcinoma,IL-22 can be secreted by infiltrated immune cells and can also be self-secreted by atypical glandular epithelium.
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