文章摘要
王爱红,朱 洁,刘翠梅,徐桂华.肌肽对局灶性脑缺血大鼠外周血IFN-γ、IL-4的影响[J].南京医科大学学报,2014,(9):1179~1183
肌肽对局灶性脑缺血大鼠外周血IFN-γ、IL-4的影响
Effects of carnosine on IFN-γ and IL-4 in peripheral blood of rats with focal cerebral ischemia
投稿时间:2014-06-06  
DOI:10.7655/NYDXBNS20140906
中文关键词: 肌肽  脑缺血  IFN-γ  IL-4
英文关键词: carnosine  cerebral ischemic  IFN-γ  IL-4
基金项目:江苏省青蓝工程项目?江苏省优势学科资助项目(YSHL0201-26)
作者单位
王爱红 南京中医药大学护理学院,江苏 南京 210023 
朱 洁 南京中医药大学护理学院,江苏 南京 210024 
刘翠梅 南京中医药大学护理学院,江苏 南京 210025 
徐桂华 南京中医药大学护理学院,江苏 南京 210026 
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中文摘要:
      目的:研究肌肽对局灶性脑缺血大鼠外周血IFN-γ?IL-4的影响?方法:将30只雄性SD大鼠随机分成假手术组(10只)?模型组(10只)和肌肽组(10只)?模型组和肌肽组以线栓法建立大鼠右侧大脑中动脉缺血模型,肌肽组于造模后按体质量1 g/(kg?d)予肌肽水溶液灌胃,其他两组予等量生理盐水灌胃?分别于术后清醒时?24 h?72 h采用Longa评分法进行神经功能评分,于术后24 h?72 h用ELISA法测定外周血IFN-γ?IL-4,于术后72 h取脑组织进行病理组织形态学观察?结果:术后72 h,肌肽组神经功能缺损评分较模型组降低(P < 0.05);术后72 h,3组外周血IFN-γ水平有明显差异(P < 0.05);术后24 h和72 h模型组外周血IFN-γ水平和IFN-γ/IL-4值明显低于假手术组(P均 < 0.05),IL-4明显高于假手术组(P < 0.05);与模型组相比,术后72 h肌肽组IFN-γ?IFN-γ/IL-4明显提高(P < 0.05),IL-4明显降低(P < 0.05);脑缺血后,神经元重度坏死,大鼠大脑皮质病理性损伤明显,肌肽处理后,神经元继发性损伤有所减轻?结论:局灶性脑缺血大鼠存在外周免疫抑制,肌肽对脑缺血大鼠外周免疫抑制有调节作用,其机制可能与提高IFN-γ?IFN-γ/IL-4值和降低IL-4有关?
英文摘要:
      Objective:To explore the effect of carnosine on peripheral blood IFN-γ and IL-4 in rats with focal cerebral ischemia. Methods:Thirty male SD rats were randomly divided into 3 groups:the sham-operated group (n=10),the model group (n=10) and the carnosine-treated group(n=10). The rat model of the right middle cerebral artery occlusion (MCAO) was established by suture method in model group and the carnosine-treated group. After making the MCAO model,the rats in the carnosine-treated group were lavaged with soluble carnosine once a day at the doses of 1 g/(kg?d) as the criteria of their body mass,and the same volume of normal saline was lavaged in the sham operation and the model group. The neurological function was scored with Longa 5-point scale at postoperative 24 h and 72 h. The levels of IFN-γ and IL-4 in peripheral blood were detected by ELISA method at 24 h and 72 h. Brain tissues were obtained for HE staining at postoperative 72 h and observed by pathological histology. Results:Compared with the model group,there were significant differences in the neurological outcome scores in the carnosine-treated group at 24 h and 72 h (P < 0.05),There was a significant decrease at 24 h and 72 h of the carnosine-treated group (P < 0.05). At 24 h and 72 h,compared with the sham-operated group,the level of IFN-γ and IFN-γ/IL-4 ratio were significant decreased (P < 0.05). The level of IL-4 was significant increased (P < 0.05). After cerebral ischemia,neurons in cerebral cortex of rats were severe necrosis and the pathological injury of cerebral cortex in rats was obvious. After carnosine treatment,secondary neuron injury mitigated. Conclusion:Peripheral immune suppression exists in rats with focal cerebral ischemia. Carnosine could has potential protective effective on peripheral immune suppression of MCAO rats and the mechanism could be IFN-γ,IL-4 and IFN-γ/IL-4 ratio elevation.
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