文章摘要
杨 晋,刘 峰,周 瑶,徐 红,张佳敏,邓 欢,雷其洪,赵德育.槐杞黄对哮喘气道及血清IL-13水平的影响[J].南京医科大学学报,2014,(9):1248~1252
槐杞黄对哮喘气道及血清IL-13水平的影响
Huaiqihuang on IL-13 expression in airway and blood in asthma
投稿时间:2014-05-07  
DOI:10.7655/NYDXBNS20140920
中文关键词: 哮喘  槐杞黄  布地奈德  IL-13
英文关键词: asthma  Huaiqihuang  budesonide  IL-13
基金项目:南京医科大学附属南京儿童医院科技发展基金(ETYY2013029)
作者单位
杨 晋 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210008淮安市妇幼保健院,江苏 淮安 223002 
刘 峰 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210009 
周 瑶 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210010 
徐 红 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210011 
张佳敏 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210012 
邓 欢 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210013 
雷其洪 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210014 
赵德育 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210015 
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中文摘要:
      目的:探讨IL-13在哮喘患儿血清中的表达及槐杞黄对于哮喘大鼠IL-13表达的可能影响及与布地奈德的共同作用?方法:选择 10例哮喘缓解期儿童 (哮喘组)及10例健康儿童 (健康对照组)为研究对象?两组年龄?性别?体质指数比较差异均无统计学意义?测试两组儿童初诊时肺功能,并利用酶联免疫吸附试验(enzymelinkedimmunosorbentassay,ELISA)检测两组儿童血清 IL-13的水平?取50只4~6周龄的健康雄性SD大鼠,适应性喂养1周后,随机分为5组,即对照组?哮喘模型组?布地奈德组?槐杞黄组?布地奈德联合槐杞黄组?用卵清白蛋白(ovalbumin,OVA)致敏及激发共持续6周,建立哮喘模型?通过HE染色观察肺部炎症细胞浸润情况?应用ELISA检测及比较各组大鼠之间肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及血清中IL-13浓度的差异?结果:与健康对照组儿童比较,哮喘组儿童初诊时第1秒用力呼气量占预测值百分比及呼气峰流速占预测值百分比均升高,血清IL-13水平升高(P < 0.05)?大鼠肺组织病理学改变:模型组气道周围大量炎症细胞浸润?柱状细胞增生?气管壁增厚;对照组则无上述表现;相比模型组,布地奈德组?槐杞黄组?布地奈德联合槐杞黄组大鼠不同程度减轻(P < 0.05)?模型组BALF及血清中IL-13的浓度增加(P < 0.05)?相较于模型组,布地奈德组?槐杞黄组?布地奈德联合槐杞黄组大鼠BALF及血清IL-13浓度有不同程度降低(P < 0.05),且布地奈德联合槐杞黄组变化比布地奈德组更明显(P < 0.05)? 结论:在OVA诱导的大鼠哮喘模型中,使用布地奈德?槐杞黄或是布地奈德联合槐杞黄治疗可以不同程度改善气道IL-13分泌,因而可能改善气道炎症?布地奈德联合槐杞黄治疗可能存在协同效应?
英文摘要:
      Objective:To evaluate IL-13 concentration changes in serum of children with asthma and the effect of Huaiqihuang on asthmatic rats for expression of inflammatory factors and the effect with budesonide. Methods:Ten asthmatic children (the asthma group) and 10 healthy children (the control group) were selected. There was no significant difference in age,gender and body mass index between the asthma group and the control group. Serum IL-13 was measured by enzyme-linked immunosorbent assay (ELISA). Fifty healthy male SD rats (aged 4-6 weeks),after adaptive feeding for one week,were randomly divided into 5 groups:the normal control group,the asthma model group,the budesonide group (the BUD group),the Huaiqihuang group (the H group) and the budesonide + Huaiqihuang group (the BUD+H group). Asthma model was induced ovalbumin (OVA) sensitization and challenge for 6 weeks. Rats were analyzed for tissue biopsy. In addition,ELISA was used to detect the IL-13 in blood and BALF in both asthmatic and control subjects. Results:Serum IL-13 concentration in the asthma group was higher than in that in the control group. Pulmonary histopathological changes:inflammatory cell infiltration,columnar cell hyperplasia,trachea wall thickening were observed in the model group around the airway;the normal group had no such differences;the BUD group,the H group,the BUD+H group showed less inflammation cells infiltration than in the model,the BUD+H group reduced more than in the H group (P < 0.05). Compared to the normal group,in the model group, IL-13 concentrations in BALF and plasma increased (P < 0.05). Compared with the model group,in the BUD group,the H group and the BUD+H group,IL-13 concentration in BALF and plasma reduced to varying degrees (P < 0.05),and the BUD+H group changed more obvious than in the BUD group (P < 0.05). Conclusion:In the OVA-induced asthma model,the use of Budesonide,Huaiqihuang or Budesonide + Huaiqihuang combination therapy can improve airway inflammation in different degrees. Budesonide + Huaiqihuang combination therapy may have a synergistic effect.
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