文章摘要
廖 胜,高 飞,葛余正,于 鹏,赵 炎,吴 然,吴剑平,贾瑞鹏.小电导型钙激活钾通道在自发性高血压大鼠阴茎海绵体中的表达[J].南京医科大学学报,2014,(10):1305~1307
小电导型钙激活钾通道在自发性高血压大鼠阴茎海绵体中的表达
SKCa in cavernous tissue of spontaneously hypertensive rats
投稿时间:2014-05-06  
DOI:10.7655/NYDXBNS20141002
中文关键词: 自发性高血压大鼠  勃起功能障碍  阴茎海绵体  钾通道蛋白
英文关键词: spontaneously hypertensive rats  erectile dysfunction  corpus cavernosum penis  potassium channel protein
基金项目:国家自然科学基金(81070597,81370853);江苏省卫生厅科教兴卫工程领军人才与创新团队科研课题(LJ201107);江苏省六大人才高峰资助项目(2011-WS-093);江苏省科教兴卫工程重点学科开放课题(KF200942)
作者单位
廖 胜 南京医科大学附属南京医院泌尿外科,江苏 南京 210006 
高 飞 南京医科大学附属南京医院泌尿外科,江苏 南京 210007 
葛余正 南京医科大学附属南京医院泌尿外科,江苏 南京 210008 
于 鹏 南京医科大学附属南京医院泌尿外科,江苏 南京 210009 
赵 炎 南京医科大学附属南京医院泌尿外科,江苏 南京 210010 
吴 然 南京医科大学附属南京医院泌尿外科,江苏 南京 210011 
吴剑平 南京医科大学附属南京医院泌尿外科,江苏 南京 210012 
贾瑞鹏 南京医科大学附属南京医院泌尿外科,江苏 南京 210013 
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中文摘要:
      目的:探讨高血压对小电导型钙激活钾通道(SKCa)在大鼠阴茎海绵体中表达的影响及意义?方法:选用同龄雄性自发性高血压大鼠(SHR)及同系正常血压大鼠(WKY)各8只,10%利多卡因麻醉后连续检测海绵体内压(ICP)及平均动脉压(MAP),并用5 V电刺激海绵体神经(CN)记录ICP/MAP值变化?运用Western blot 和逆转录-聚合酶链式反应(RT-PCR)技术检测阴茎海绵体组织中SKCa蛋白和mRNA表达?结果:5 V电刺激后SHR组ICP/MAP值显著低于WKY组(0.31 ± 0.04 vs 0.76 ± 0.06),SKCa蛋白和mRNA的表达在SHR组(0.18 ± 0.01?0.39 ± 0.01)较WKY组(0.49 ± 0.02?0.81 ± 0.08)明显降低,差异具有统计学意义(P < 0.05)?结论:高血压可以降低大鼠勃起功能,这可能与自发性高血压大鼠阴茎海绵体组织中SKCa表达下降密切相关?
英文摘要:
      Objective:To validate the effect of hypertension on small conductance calcium-activated potassium channels in cavernous tissue of spontaneously hypertensive rats. Methods: Male spontaneously hypertensive rats (SHR) (n = 8) and normotensive Wistar Kyoto rats (WKY) (n = 8) were selected. A series of electric stimulation were utilized to the corpus cavernosum nerves of rats, the changes of ICP /MAP were observed repeatedly, and the expressions of SKCa protein and mRNA were assessed by Western blotting and RT-PCR. Results: Compared with the WKY group, ICP /MAP and the expression of SKCa gene mRNA and protein were significantly lower in the SHR group (P < 0.05), and the expression of SKCa protein and mRNA was significantly lower in the SHR group in comparison with that of the WKY group (P < 0.05). Conclusion: Erectile dysfunction in hypertension is associated with the low expression of SKCa in the cavernous tissue of spontaneously hypertensive rats.
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