文章摘要
朱洁晨,沈吉凤,朱述阳,石瑞瑞,陈 琳.HIF-1α在低氧刺激的气道平滑肌细胞中的表达及意义[J].南京医科大学学报,2014,(10):1308~1312
HIF-1α在低氧刺激的气道平滑肌细胞中的表达及意义
Expression of HIF-1α on airway smooth muscle cells suffering from hypoxia and related clinical importance
投稿时间:2014-03-04  
DOI:10.7655/NYDXBNS20141003
中文关键词: 低氧  气道平滑肌细胞  HIF-1α
英文关键词: hypoxia  airway smooth muscle cell  HIF-1α
基金项目:徐州市科技局资助项目(XM098077)
作者单位
朱洁晨 徐州医学院附属医院呼吸内科,江苏 徐州 221002 
沈吉凤 大屯煤电集团责任(有限)公司中心医院内科,江苏 徐州 221611 
朱述阳 徐州医学院附属医院呼吸内科,江苏 徐州 221002 
石瑞瑞 徐州医学院附属医院呼吸内科,江苏 徐州 221003 
陈 琳 徐州医学院附属医院呼吸内科,江苏 徐州 221004 
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中文摘要:
      目的:观察低氧状态下大鼠气道平滑肌细胞(airway smooth muscle cell,ASMC)增殖情况及低氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)表达变化,探讨ASMC低氧反应机制?方法:体外传代(5代)培养大鼠ASMC分3组:常氧状态下培养为正常对照组(A组),低氧状态下培养为单纯低氧组(B组),低氧 + HIF-1α抑制剂2-甲氧雌二醇(2-methoxyestradiol,2ME)为低氧2ME干预组(C组)?甲基甲苯基硫(MTS)法检测细胞增殖,荧光实时定量RT-PCR检测HIF-1α mRNA表达,Western blot检测HIF-1α蛋白表达?结果:①大鼠ASMC增殖B组比A组更加明显(1.123 ± 0.006 vs 0.809 ± 0.003,P < 0.05),C组较B组明显受抑制(1.012 ± 0.005 vs 1.123 ± 0.006,P < 0.05);②HIF-1α mRNA和蛋白表达B组比A组表达明显增加(5.265 ± 0.040 vs 0.449 ± 0.017,P < 0.05和0.905 ± 0.013 vs 0.105 ± 0.008,P < 0.05),C组较B组表达明显减少(1.903 ± 0.044 vs 5.265 ± 0.040,P < 0.05和0.278 ± 0.012 vs 0.905 ± 0.013,P < 0.05)?ASMC增殖与HIF-1α mRNA表达呈正相关(r = 0.850),与HIF-1α蛋白表达呈正相关(r = 0.883)?结论:低氧刺激促进大鼠ASMC增殖,可能是由HIF-1α所介导?
英文摘要:
      Objective:To explore whether hypoxia-inducible factor-1α(HIF-1α)mediates hypoxic proliferation in airway smooth muscle cells(ASMC). Methods:Rat ASMC were incubated in vivo and grouped in three:ASMC that is cultured under normoxia was defined as control group(A group),ASMC that is cultured under hypoxia was defined as pure hypoxia group(group B),ASMC that is cultured under hypoxia and pretreated with 2-methoxyestradiol(2ME),which is inhibitor of HIF-1α,was defined as hypoxia and 2ME pretreatment group(group C). Proliferation of ASMC was evaluated by MTS. Real-time PCR and Western blot were used to analyze expression of HIF-1α mRNA and protein. Results:①The proliferation of rats ASMC is significantly higher in group B than that in group A(1.123 ± 0.006 vs 0.809 ± 0.003,P < 0.05),but it is significantly lower in group C than that in group B(1.012 ± 0.005 vs 1.123 ± 0.006,P < 0.05). ②Expression of HIF-1α mRNA and protein increased significantly in group B than that in group A(5.265 ± 0.040 vs 0.449 ± 0.017,P < 0.05 and 0.905 ± 0.013 vs 0.105 ± 0.008,P < 0.05),and decreased significantly in group C than that in group B(1.903 ± 0.044 vs 5.265 ± 0.040,P < 0.05 and 0.278 ± 0.012 vs 0.905 ± 0.013,P < 0.05),expression of HIF-1α mRNA(r = 0.850)and protein(r = 0.883) in ASMC correlated significantly with proliferation. Conclusion:HIF-1α mediated rat ASMC proliferation under hypoxia.
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